수혈 전 검사 장비인 AutoVue Innova와 Techno TwinStation의 평가 = Evaluation of Two Automated Instruments for Pre-transfusion Testing: AutoVue Innova and Techno TwinStation

다국어 초록 (Multilingual Abstract)

Background : Despite the advances in total laboratory automation, a considerable amount of work
in blood banks is still done using outdated manual methods. Some automated pre-transfusion testing
instruments have recently been developed. Of these, we evaluated and compared the AutoVue
Innova (Ortho, USA) and the Techno TwinStation (DiaMed AG, Switzerland).
Methods : Forward and reverse ABO/Rh typing and unexpected antibody screening and identification
tests were performed on 4,628 samples using the manual method and the two automated
instruments. Two different anticoagulants (EDTA and citrate) were compared in ABO/Rh typing and
unexpected antibody screening tests. Titrating studies were conducted on the following 7 dilutions
using 5 samples of irregular antibodies with anti-E, anti-E & -c, anti-D, and anti-Lea with anti-Fya: 1:2,
1:4, 1:8, 1:16, 1:32, 1:64, and 1:128. The test throughput per hour, the time required to perform 1
and 100 tests, and a simulation test for total events occurring in 1 day were also measured.
Results : No erroneous results were reported between the two instruments and the manual method.
Discrepancies observed in 10 cases (0.4%) of ABO/Rh typing were of higher intensity with AutoVue
Innova than with the manual method. AutoVue Innova exhibited the highest sensitivity in the titrating
study and throughput performance compared with the manual method and the Techno TwinStation.
Especially in the throughput and time required to complete 100 antibody screening tests, AutoVue
Innova had a 3.3- and 3.5-fold higher performance, respectively, than Techno TwinStation.
Conclusions : Because both of the two fully automated instruments (AutoVue Innova and Techno
TwinStation) had high levels of accuracy and performance, it is expected that use of fully automated
instruments will reduce human labor, turnaround time, and operator error in the blood bank. (Korean
J Lab Med 2008;28:214-20)

다국어 초록 (Multilingual Abstract)

Background : Despite the advances in total laboratory automation, a considerable amount of work
in blood banks is still done using outdated manual methods. Some automated pre-transfusion testing
instruments have recently been developed. Of these, we evaluated and compared the AutoVue
Innova (Ortho, USA) and the Techno TwinStation (DiaMed AG, Switzerland).
Methods : Forward and reverse ABO/Rh typing and unexpected antibody screening and identification
tests were performed on 4,628 samples using the manual method and the two automated
instruments. Two different anticoagulants (EDTA and citrate) were compared in ABO/Rh typing and
unexpected antibody screening tests. Titrating studies were conducted on the following 7 dilutions
using 5 samples of irregular antibodies with anti-E, anti-E & -c, anti-D, and anti-Lea with anti-Fya: 1:2,
1:4, 1:8, 1:16, 1:32, 1:64, and 1:128. The test throughput per hour, the time required to perform 1
and 100 tests, and a simulation test for total events occurring in 1 day were also measured.
Results : No erroneous results were reported between the two instruments and the manual method.
Discrepancies observed in 10 cases (0.4%) of ABO/Rh typing were of higher intensity with AutoVue
Innova than with the manual method. AutoVue Innova exhibited the highest sensitivity in the titrating
study and throughput performance compared with the manual method and the Techno TwinStation.
Especially in the throughput and time required to complete 100 antibody screening tests, AutoVue
Innova had a 3.3- and 3.5-fold higher performance, respectively, than Techno TwinStation.
Conclusions : Because both of the two fully automated instruments (AutoVue Innova and Techno
TwinStation) had high levels of accuracy and performance, it is expected that use of fully automated
instruments will reduce human labor, turnaround time, and operator error in the blood bank. (Korean
J Lab Med 2008;28:214-20)

참고문헌 (Reference)

• 1 Han KS, "Transfusion medicine" Korea Medical Book Pubisher 360-, 2006
• 2 Burtis CA, "Tietz textbook of clinical chemistry and molecular diagnostics" Saunders 492-493, 2006
• 3 Han KS, "Irregular blood group antibodies in Korean" 24 : 145-153, 1989
• 4 Mcpherson RA, "Henry’s clinical diagnosis and management by laboratory methods" Saunders 58-, 2007
• 5 Morelati F, "Evaluation of a new automated instrument for pretransfusion testing" 38 : 959-965, 1998
• 6 Recommendations for evaluation, validation and implementation of new techniques for blood grouping, antibody screening and cross-matching, "British Committee for Standards in Haematology, Blood Transfusion Task Force" 5 : 145-150, 1995