커피박 첨가에 의한 새송이버섯 균사체의 생육 및 생리활성 증가 = Enhancement of Growth and Bioactivity of Pleurotus eryngii Mycelia by Spent Coffee Ground

다국어 초록 (Multilingual Abstract)

Pleurotus eryngii. one of the most popular edible mushrooms, has been well known for its biological activities such as antioxidation, antitumor and immune modulation. Spent coffee ground(SCG) that is a waste product from the coffee industry has been continuously investigated for its reutilization. In this study, SCG was added to the fungal cultuvation medium and analyzed for its effect on the growth and physiological activity of P. eryngii mycelia. It was clearly demonstrated that SCG could accelarate mycelia growth. 1% SCG culture was very notable by showing 2.5-fold higher dry cell weight comapred to the control culture, which suggested SCG as an excellent activator for the growth of P. eryngii mycelia. By the addition of SCG, polyphenol content was increased by two fold but there was no change in polysaccharide content. In the analysis of DPPH scavenging activity, SCG was determined as a valuable source in order to significantly increase the antioxidative activity of the mycelium.

국문 초록 (Abstract)

새송이버섯(Pleurotus eryngii)은 인기 있는 식용버섯 중의 하나로서 항산화, 항암 또는 면역조절 기능 등의 인체에 유익한 생리활성 기능을 지닌다. 본 연구에서는 새송이버섯 균사체 배양 시 커피음료 생산 과정으로부터 폐기되는 커피박(spent coffee ground: SCG) 첨가에 의한 균사체의 생장과 기능성의 변화를 조사하였다. 그 결과, SCG(1-10%, w/v) 첨가 시 생장속도가 상당히 증가하는 경향을 나타내었다. 특히 1% SCG 첨가 시 무첨가에 비해 건조중량이 2.5배 증가함으로써 SCG가 탁월한 새송이버섯 균사체의 생장촉진제 기능이 제시되었다. SCG 첨가에 의하여 균사체의 polysaccharide 함량은 변화가 없었으나 polyphenol량 및 항산화능의 증대를 확인할 수 있었다.

참고문헌 (Reference)

• 1 Estrada, R. A. E, "Yield, size and bacterial blotch resistance of Pleurotus eryngii grown on cottonseed hulls/oak sawdust supplemented with manganese, copper and whole ground soybean" 98 : 1898-1906, 2007
• 2 Hatano, T, "Two new flavonoids and other constituents in licorice root: their relative astringency and radical scavenging effects" 36 : 1090-1097, 1988
• 3 Silva, M. A, "The use of biomass residues in the Brazilian soluble coffee industry" 14 : 457-467, 1998
• 4 Tokimoto, T, "Removal of lead ions in drinking water by coffee grounds as vegetable biomass" 281 : 56-61, 2005
• 5 Wasser, S. P, "Medicinal properties of substances occurring in higher Basidiomycetes mushrooms: Current perspectives (review)" 1 : 31-62, 1999
• 6 Franca, A. S, "Kinetics and equilibrium studies of methylene blue adsorption by spent coffee grounds" 249 : 267-272, 2009
• 7 Simoes, J, "Immunostimulatory properties of coffee mannans" 53 : 1036-1043, 2009
• 8 Vennat, B, "Hamamelis virginiana: identification and assay of proanthrocyanidins, phenolic acids and flavonoids in leaf extracts" 67 : 11-14, 1992
• 9 Machado, E. M. S, "Growth of fungal strains on coffee industry residues with removal of polyphenolic compounds" 60 : 87-90, 2012
• 10 Esquivel, P, "Functional properties of coffee and coffee by-products" 46 : 488-495, 2012
• 11 Liu, X, "Extraction and antioxidant activities of intracellular polysaccharide from Pleurotus sp. mycelium" 47 : 116-119, 2010
• 12 Liu, K, "Evaluation of three composting systems for the management of spent coffee grounds" 102 : 7966-7974, 2001
• 13 Minamisawa, M, "Determination of biologically active substances in roasted coffees using a diode-array HPLC system" 20 : 325-328, 2004
• 14 Hecimovic, I, "Comparative study of polyphenols and caffeine in different coffee varieties affected by the degree of roasting" 129 : 991-1000, 2011
• 15 Saenger, M, "Combustion of coffee husks" 23 : 103-121, 2001
• 16 Nkondjock, A, "Coffee consumption and breast cancer risk among BRCA1 and BRCA2 mutation carriers" 118 : 103-107, 2006
• 17 Ridha, H, "Co-composting of spent coffee ground with olive mill wastewater sludge and poultry manure and effect of Trametes versicolor inoculation on the compost maturity" 88 : 677-682, 2012
• 18 Niseteo, T, "Bioactive composition and antioxidant potential of different commonly consumed coffee brews affected by their preparation technique and milk addition" 134 : 1870-877, 2012
• 19 Nebesny, E, "Antioxidative activity of green and roasted coffee beans as influenced by convection and microwave roasting methods and content of certain compounds" 217 : 157-63, 2003
• 20 Palacios, I, "Antioxidant properties of phenolic compounds occuring in edible mushrooms" 128 : 674-678, 2011
• 21 Becker, E. M, "Antioxidant evaluation protocols: Food quality or health effects" 219 : 561-571, 2004
• 22 Vignoli, J. A, "Antioxidant activity, polyphenols, caffeine and melanoidins in soluble coffee: The influence of processing conditions and raw material" 124 : 863-868, 2011
• 23 Sung, W. S, "Antifungal action of chlorogenic acid against pathogenic fungi, mediated by membrane disruption" 82 : 219-226, 2010
• 24 Singleton, V. L, "Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent" 229 : 152-178, 1999
• 25 Buyssee, J, "An improved colorimetric method to quantify sugar content of plant tissue" 44 : 1627-1629, 1993