Affinity purification in target identification: the specificity challenge

다국어 초록 (Multilingual Abstract)

Since phenotype-based screening directlyevaluates capability of small molecules for modulatingbiology in actual biological systems, it has become animportant discover modality in modern pharmaceuticalsciences. However, in order to fully elucidate the molecularmechanism underlying the bioactivity of small molecules,identification of their biological targets is an indispensablestep. Among the many target identification strategiesdeveloped during the past several decades, affinity purificationremains to be one of the most important and powerfulapproaches, as it can directly reveal the physicalinteractions between small molecules and their biomoleculartargets. However, due to the complexity of the proteomeand the diversity of small molecule–proteininteractions, affinity purification faces the specificitychallenge: how to identify the true specific targets from thenon-specific background? Focusing on this challenge, inthis review, we briefly introduce the history and backgroundof affinity purification, and then we discussed themajor technological developments aiming to address thischallenge. We have summarized these approaches in twocategories: noise reduction and comparative distinction.
This review also highlights the importance of choosing anintegrated approach combining multiple methods toachieving success in target identification.

참고문헌 (Reference)

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