돼지 난자의 직경이 체외성숙 및 체외발달에 미치는 영향 = Porcine Oocyte Diameter in Relation to Maturational and Development Competence

국문 초록 (Abstract)

도축장에서 채취한 돼지난자를 직경별로 간격으로 나누어, 난자의 크기에 따른 체외성숙과 발육능을 구명코자 체외성숙과 체외수정 후의 배 발달율을 조사하였다. 채취한 난자의 투명대를 제외한 평균직경은 이었으며, 직경별 분포는 < 105, 105~110, 110~115,115~120, 120~125, <이 각각 3.0, 11.0, 31.2, 41.5, 13.0 및 0.4%로 72.7%가 110부터 사이였다. 체외성숙율에 있어서 직경 미만난자는 66.7%인 반면

다국어 초록 (Multilingual Abstract)

To investigate the maturational and development competece of porcine oocytes of different diameter groups, oocytes were obtained by aspiration from slaughterdhouse ovaries. After washing three times in NCSU23 medium, each cumulus-oocyte complex was transferred into a drop of the maturation medium (one oocyte per drop) under paraffin oil. The diameter without zona pellucida of oocytes was measured with micor-calibrator (Mikrometer, E. Leitz) on a screen connected to a VCR on an inverted microscope . After being measured, the oocytes were divided into 6 groups according to their diameter size : <105, 105 to < 110, 110 to < 115, 115 to < 120, 120 to < 125 and > , and in vitro maturation (IVM), fertillzation (IVF) and production (IVP) of oocytes / embryo was performed. The rates of in vitro maturation on oocytes in the greater 105 size groups(91.8~100%) were significantly (P<0.05) higher than in the < 105 group(66.7%). The rates of sperm penetration were significantly (P<0.05) low in < group (50.0%) than others groups (81.6~85.5%). But the plyspermic fertilization rate was significantly (P<0.05) higher in < oocytes groups than in the size groups. The rates of cleavage and development to blastocysts rose as oocytes diameter increased, however, while oocytes over in diameter failed to develop to blastocysts. There results suggest that porcine oocytes have acquired full meiotic competece at a diameter of but not yet attended full development competence to blastocyst and that oocytes have acquired full development competence at a diameter of .