Saccharomyces cerevisiae deletion mutant의 세라마이드 생합성

국문 초록 (Abstract)

Saccharomyces cerevisiae의 deletion mutant를 이용하여 ydc1, ypc1, scs7, sur1, csg2, ipt1, lcb4, lcb5, dpl1의 deletion이 세라마이드의 생산에 미치는 영향을 고찰하였다. 세라마이드는 ELSD가 연결된 HPLC를 통하여 분석하였으며 △ydc1 mutant의 세라마이드 생산량이 6 mg ceramide/g cell로 최대량을 나타내었으며 △sur1 mutant, △lcb5 mutant, △dpl1 mutant의 경우 control로 사용한 BY4742와 비슷한 세라마이드 생산량을 나타내었고, 그 외 △ypc1 mutant, △scs7 mutant, △csg2 mutant, △ipt1 mutant, △lcb4 mutant는 BY4742보다 낮은 세라마이드 생산량을 나타내었다.

다국어 초록 (Multilingual Abstract)

Ceramide is important not only for the maintenance of the barrier function of the skin but also for the water-binding capacity of the stratum corneum. Though the effectiveness of ceramide is not understood fully, ceramide has become a widely used ingredient in cosmetic and pharmaceutical industries. However, ceramide production from Saccharomyces cerevisiae has not been widely studied and the quantity are very low. Gene deletion in the cell is used frequently to investigate the function of gene and verification research of drug target. Specially, deletion mutant library is useful for a large amount functional analysis of gene. In this study, deletion mutants of genes on the metabolic pathway of ceramide synthesis in S. cerevisiae were grown in a batch culture and the cellular content of ceramide was measured. The ceramide content was highest in Δydc1 mutant and 6 mg ceramide/g cell was obtained.

목차 (Table of Contents)

• Abstract
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• REFERENCES

참고문헌 (Reference)

• 1 "http://pathway.yeastgenome.org/"
• 2 Thomas, J. M., "The separation and direction detection of ceramide and sphingoid bases by normal-phase high performance liquid chromatography and evaporative light-scattering detection" 276 : 242-250, 1999
• 3 Nagiec, M. M., "The LCB4(YOR171c) and LCB5(YLR260w) genes of Saccharomyces encode sphingoid long chain base kinases" 273 (273): 17437-19442, 1998
• 4 Lee, J. H., "Systems Biotechnology" 38 : 10-15, 2004
• 5 Stock, S. D., "Syringomycin E Inhibition of Saccharomyces cerevisiae: Requirement of Biosynthesis of Sphingolipids with Very-Long-Chain Fatty Acids and Mannose- and Phosphoinositol- Containing Head Groups" 44 : 1174-1180, 2000
• 6 Im, D. S., "Sphingosine, Sphingosine kinase and Apoptosis" 24 (24): 38-72, 2004
• 7 Cuvillier, O., "Sphingosine in apoptosis signaling" 1585 (1585): 153-162, 2002
• 8 김세경, "Saccharomyces cerevisiae 의 회분식 배양에서 세라마이드의 생산" 한국생물공학회 23 (23): 449-451, 2008
• 9 Beeler, T. J., "SUR1(CSG1/BCL21), a gene necessary for growth of Saccharomyces cerevisiae in the presence of high Ca2+ concentrations at 37℃, is required for mannosylation of inositolphosphorylceramide" 255 : 570-579, 1997
• 10 류경호, "Mobile Phase Compositions for Ceramide III by Normal Phase High Performance Liquid Chromatography" 한국생물공학회 9 (9): 47-51, 2004
• 11 Hannum, Y. A., "Lysosphingolipid inhibit protein kinase C: implications for the sphingolipidoses" 235 (235): 670-674, 1987
• 12 Lynch, K. R., "Life on the edg" 20 (20): 473-475, 1999
• 13 Funato, K., "Lcb4p is a key regulator of ceramide synthesis from exogenous long chain sphingoid base in Saccharomyces cerevisiae" 278 (278): 7325-7334, 2003
• 14 Rupeic, J., "Isolation and chemical composition of the ceramide of the Candida lipolytica yeast" 91 : 153-161, 1998
• 15 Kim, D. U., "Current progress of the genome-wide deletion project in the fission yeast, Schizosaccharomyces pombe and its application: functional genomics and HCS drug target screening, In Bioscience, Promise for the future" 173-, 2006
• 16 Mao, C, "Cloning of an Alkaline Ceramidase from Saccharomyces cerevisiae" 275 (275): 6876-6884, 2000
• 17 Mao, C, "Cloning and Characterization of a Saccharomyces cerevisiae Alkaline Ceramidase with Specificity for Dihydroceramide" 275 (275): 31369-31378, 2000
• 18 Han, S. J., "Bioinformatics for comparative genomics study of S. pombe, In Bioscience, Promise for the future" 173-, 2006