Screening of Resistance Genes Linked to Brown Planthopper Using STS Marker in Aromatic Rice Germplasm

다국어 초록 (Multilingual Abstract)

Brown planthopper (BPH) is a serious insect pest of rice crop throughout rice growing countries, and yield loss due to its infection can be up to 60%. This study aimed to evaluate efficiency of molecular markers for screening BPH resistance accessions among 86 aromatic rice germplasm. Eighty-six accessions of aromatic rice germplasm included two accessions of Tongil type (bred in Korea), 28accessions of japonica type and 56 accessions of indica type. We applied eight STS markers (pBPH9, pBPH19,pBPH20, pBPH21, AJ09-b, RG457L, RG457B, and 7312.T4A)which were linked to four of BPH resistance genes, Bph1,Bph13(t), Bph10, and Bph18(t) respectively. One japonica type accession, 415XIr352, and six indica type accessions possessed one or four positive bands when tested with four STS markers linked to Bph1 gene. One indica type aromatic rice, Basmati9-93, showed the target bands linked to the Bph10 gene. The other accessions did not show same fragments as the respective resistant lines. Bph13(t) is the most widely introduced resistance gene and only one accession showed positive bands implying that this accession might harbor Bph10 and Bph18(t) genes. Three aromatic accessions, Domsiah, Khao Dawk Mali 105 and 415XIr352showed gene pyramiding of Bph1 and Bph13(t). Two indica aromatic rice, Ds 20 and Basmati 9-93, possessed at least two BPH resistance genes, Bph1, Bph18(t) and Bph13(t),Bph18(t), respectively. These results indicates that aromatic rice germplasm have narrow diversities of BPH resistance genes.

참고문헌 (Reference)

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