비타민나무 잎 에탄올추출물의 AMPK 활성화를 통한 3T3-L1 지방전구세포의 adipogenesis 억제효과 = Ethanol Extract of Hippophae Rhamnoides L. Leaves Inhibits Adipogenesis through AMP-activated protein kinase (AMPK) Activation in 3T3-L1 Preadipocytes

국문 초록 (Abstract)

본 연구는 HRL의 3T3-L1 지방전구세포의 분화과정 중에HRL이 지방의 축적에 미치는 영향을 확인하였다. MTT assay 를 이용하여 세포 독성을 측정한 결과 100 ㎍/㎖의 농도에서도세포증식에 영향을 미치지 않는 것을 확인하였고, 이와 같은 결과를 토대로 Oil Red O 염색법을 이용하여 지방세포 분화 억제능을 측정하였다. 그 결과, HRL의 경우 100 ㎍/㎖의 농도에서82.25% 지방 축적 억제능을 나타내었다. 지방생성에 영향을 미치는 유전자 발현량을 측정하기 위해 RT-PCR법과 western blot법을 시행하였다. HRL은 SREBP-1c, PPARγ와 C/EBPα의mRNA 발현을 억제시켰고, 지방생성에 영향을 미치는 효소인FAS의 생성을 조절하는 것으로 나타났다. 또한, HRL 처리로AMPKα의 단백질 발현이 증가하였으며, PPARγ의 발현량이 감소하는 것을 확인하였다. 이상의 결과들로부터 HRL은 AMPKα 의 활성화를 통한 지방 합성을 억제를 보유하고 있는 바, 향후항비만 기능성 소재로 활용될 수 있을 것으로 생각한다

다국어 초록 (Multilingual Abstract)

In the present study, we investigated the effect of 70% EtOH extract from Hippophae Rhamnoides L. leaves (HRL) on the anti-obesity effect in 3T3-L1 cells. The effects of HRL on lipid accumulation in 3T3-L1 cells were examined using Oil Red O staining. In addition, we examined the gene expression levels by using RT-PCR and western blot. The results of this analysis showed that 100 ㎍/㎖ HRL significantly increased the inhibition of lipid accumulation by 82.25%; significantly decreased the mRNA expression of sterol regulatory element binding protein-1c (SREBP-1c), peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding proteins α (C/EBPα), and fatty acid synthase (FAS) in 3T3-L1 cells as well as the stimulated protein expression of AMP-activated protein kinase (AMPK); and suppressed the expression level of PPARγ. These results suggest that HRL can prevent adipogenesis through activation of AMPKα and inhibition of adipogenesis transcription factors.

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