담도ㆍ췌장 : 만성 췌장염과 췌장암에서 면역조직화학염색법을 통한 Ki - 67 , p53 , K - ras 단백 발현에 대한 연구 = Immunohistochemical Evaluation of Ki - 67 , p53 , K - ras Expression in Chronic Pancreatitis and Pancreatic Adenocarcinoma

다국어 초록 (Multilingual Abstract)

Background/Aims: Hyperplasia or dysplasia of pancreatic duct epithelium has been frequently found in the pancreas of pancreatic cancer or chronic pancreatitis. We hypothesized that ductal hyperplasia and dysplasia in the pancreas represent precursor lesions of cancer. Methods: We examined the expression of Ki-67, CEA, p53, K-γαs using the methods of H&E staining and immunohistochemical staining for 11 pancreatic cancer and 12 chronic pancreatitis specimens. For the specimens of pancreatic cancer, we classified the tissue into normal (n=7), ductal hyperplasia (n=3), dysplasia (n=4), and cancer lesion (n=ll). The tissue of chronic pancreatitis were also divided into normal (n=10), ductal hyperplasia (n=4), and dysplasia (n=5). Results: In pancreatic cancer, the Ki-67 index was 3.73±3.58 in normal site, 6.62±4.39 in ductal hyperplasia, 13.47±4.02 in dysplasia, and 37.03±10.05 in cancer tissue (p<0.05). In this study, the expression of p53 protein was observed in 78% of dysplasia and 91% of carcinoma cells but not in normal ducts and ductal hyperplasia. K-γαs was expressed in 33% of ductal hyperplasia, 67% of dysplasia, and 80% of cancer tissue but not in normal ducts. Conclusions: These data support the hypothesis that ductal hyperplasia and dysplasia of the pancreas represent precursor lesions of cancer. These results need further evaluation of molecular study of oncogenes. (Korean J Gastroenterol 2001;37:370-378)