담도폐색 흰쥐에서 Cholic Acid와 Phenobarbital 투여 대한 Hepatic Microsomal Cytochrome P-450 Apoprotein의 변화 = Qualitative Alteration in Hepatic Microsomal Cytochrome P-450 Apoproteins Associated with Bile Duct Ligation, and the Administration of Cholic Acid and Phenobarbital

다국어 초록 (Multilingual Abstract)

We have investigated in rat liver whether different forms of cytochrome P-450 are alterated in hepatic disorders associated with impaired drug metabolism. Total hepatic cytochrome P-450 is decreased after either bile duct ligation or the administration of estradiol. In contrast, phenobarbital administrated alone increase hepatic content of cytochrome P-450, and when administrated with estradiol the reduction in cytochrome P-450 was prevented.
Four forms of microsomal cytochrome P-450 apoproteins, ranging in molecular weight from 50 000 to 58,000, were tentatively identified in a sodium dodecyl sulfate(SDS)-6M urea polyaocrylamide gel electrophoresis system. Phenobrbital administration icreased primarily band Ⅳ(50,000 daltons). Bile duct ilgation was associated with a marked reduction in bands Ⅱ and Ⅲ while bands Ⅱ and Ⅲ were decreased with estradiol benzoate administration. Sumultaneous administration of phenobarbital and estradiol demonstrated a return of band Ⅰ and an increase in density of bands Ⅲ and Ⅳ. Simultaneous administration of cholic acid and estradiol demonstrated a return of band Ⅰ and not altered in band Ⅲ and Ⅳ.
These studies support the hypothesis that multiple forms of cytochrome P-450 are present in liver microsomal membranes and that alterations in spenific apoproteins may be associated with an increase or a decrease in the functional properties of cytochrome P-450.