양혈장근건보탕(養血壯筋健步湯)과 전침의 병용치료가 손상된 척수신경 및 운동기능에 미치는 영향 = Effects of Yanghyuljanggeungunbo-tang(Yangxuezhuangjinjianbutang) and Electrical Acupuncture on the Spinal Nerve Injury and the Motor Function

다국어 초록 (Multilingual Abstract)

Objectives: The purpose of this study was to investigate the effects of Yanghyuljanggeungunbo-tang(Yangxuezhuangjinjianbu-tang, YGKT) and electrical acupuncture treatment in spinal cord injury(SCI)-induced rats. Methods: The subjects were divided into 5 groups ; Normal, Control-no treatment after SCI, Experimental 1(Exp. 1)-taken with YGKT 500 ㎎/㎏ 0.5 ㎖ daily after SCI. Experimental 2(Exp. 2)-taken with electrical acupuncture after SCI and Experimental 3(Exp. 3)-taken with YGKT 500 ㎎/㎏ 0.5 ㎖ and electrical acupuncture after SCI. After each operation, the present author observed cytological changes, the motor behavior recovery and nerve regeneration by analysis of the motor behavior tests, EMG, hematological(AST, ALT, WBC), histological and immunological changes. Rats were tested by Motor behavior test at 1st, 3rd, 7th, 14th and 21st day. Results: 1. All the experimental groups were improved compared with control group in the motor behavior tests including Tarlov test, Basso-Beattle-Bresnahan locomotor rating scale, modified inclined plane test, open field test, grid walk test and narrow beam test. Especially Exp. 3 was significantly improved among other groups. 2. In EMG test, H and M wave were significantly increased in Exp. 3. 3. All the experimental groups were significantly decreased compared with control group in AST, ALT and WBC. 4. NGF, BDNF and Trk B of spinal cord gray matter in all the experimental groups were increased compared with control group. Especially, Exp. 3 was more effective. 5. In histological observations, muscle contraction and denaturation of gastrocnemius muscle of all the experimental groups were inhibited. Especially, those of Exp. 3 was more effective. On the observations of liver and kidney, cell atrophy and apoptosis of all the experimental groups were decreased compared with control group. Especially, those of Exp. 3 was more effective. Conclusions: It can be suggested that YGKT and electrical acupuncture may improve motor behavior, EMG, hematological, histological and immunological findings in SCI-induced rats. Especially, combination of these two treatments will be somewhat better in spinal nerve recovery and motor function improvement.