Hexachlorophene, Dithiazaine, Fuadin, Chloroquine 및 Gentian violet 投與後 肝吸蟲 蟲體의 變化에 關한 病理組織學 및 組織化學的 硏究 = Studies on histological and histochemical changes in adult worms of Clonorchis sinensis treated with Hexachlorophene, Dithiazanine iodide, Faudin, Chloroquine, and Gentian violet

다국어 초록 (Multilingual Abstract)

Clonorchiasis has been known to be one of the most important prarasitic disease in the Asiatic nations including korea,Japan, China, Formosa, Thailand, etc. The etiologic agent, Clonorchis sinensis was first discovered by McConnel in 1874, and the treatment for this infection was first tried by Brug in 1919 with an antimony compound. A few other drugs have been tried, but so far no reliable therapeutic agent has been found for clonorchiasis. In 1960 sinensis using some 60 different chemicals and found Dithiazanine iodide to be best in clonorchicidal effect. In 1962 Takagi Studied on the histological changes in adult worms of Clonorchis sinensis treated with antimony preparatiion and Dithiazanine iodide and demonstrated both the antimony preparation and Dithiazanine iodide to have high cloorchicidal activity, but their modes of action are fundamentally different from each other. More recently Kim, at al. and Chung have found hexachlorophene to have the best clonorchicida effect in test tube and animal experiments, and further found it to be a very promising drug for the clinical treatment of human cases of clonrchiasis.
In order to find any histological and histochemical changes in the body of Cloorchis sinensis from the use of various drugs used as clonorchicides I have carried out the following studies.
Materials and methods;
1. Thirty healthy rabbits weighing 1.5 to 2.0㎏ were divided into six groups of five each, and each of the five groups was given about 300 to 500 of active metacercariae per os, and one group was set aside as a control.
2. In 60 days all the rabbits passed the eggs in teh excrements showing the infection.
3. Each group was given the following drugs, hexachlorphene 10㎎ per ㎏ of body weight, dithiazanine iodide 30㎏ per ㎏ of body weight, faudin 0.1㎖ per ㎏ of body weight, chloroquine diphosphate 50㎎ per ㎏ of body weight, and gentian violet 10.㎎ per ㎏ of body weight respectively daily for six days.
4. On the seventh day the animals were killed, and the adult worms were collected from the liver.
5. the worms were properly fixed in Carnoy's solution and 10% buffer formal water, and were embeded in usual manner for a serial sections in 6μthichness.
The staining methods used for the experiments were as follows:
1. Hematoxyline-Eosin method.
2. Iron-hematoxylin method.
3. Carbohydrates
a. The Periodic acid Schiff(PAS) reaction(McMannus method)
b. PAS reaction after salivary digestion.
4. Nucleic acids
a. Feulgen reaction
b. Taft method
c. Methylgreen-Pyronin method
5. Lipase
a. Gomori-Tween method.
Results:
Hexachlorophene was given to the experimental rabbit chlornorchiasis and the changes in the body of the worm was carefully observed hsitologically and histochemically and the changes were also compared with those present from the use of the drugs such as dithiazanine iodide, faudin, chloroquine diphosphate, and gentian violet.
1 Group A with hexachlophen; Most of the changes were presnt in the sustentacular tissue, with hyaline degeneration. The amount of polysaccharides, glycogen and RNA were decreased. There were also granular degeneration in the digestive and excretory bladder and vitelline glands. In uterus there were hyaline degeneration and a few deformed eggs.
2. Group B with dithiazanine iodid;ln sustenticular tissue there was hyaline degeneration, but in the intesine and excretory bladder and in the uterus there was hyaline degeneration, and many more deformed eggs were found.
3. Group C with faudin; In the sustenticular tissue there was hyaline degeneration, but in a lesser degree. In intestine and excretory bladder there was a change similar to the Group A. In teh uterus there was a hyaline degeneration and the eggs were mostly deformed and much decreased in number. In this group most of teh changes are seen in the reproductive system
4. Group D with chloroquine diphsophate; The changes in sustenticular tissue, the sucker and intestine, and excretory bladder were similar to those of Group C, but in a lesser degree. There were a hyaline degeneration in subcuticular tissue and granular degeneration in the parenchyma cells. There was also granular degeneration in the sucker and vittelline glands. The intestine and exretory bladder and uterus were stained very poorly.
There were also many deformed eggs.
5. Group E with gentian violet; The changes in the sustenticular tissues was very similar to the Group D, and the subduticular tissue showed hyaline degeneration, and the parenchyma cells showed granular degeneration. The cuticules were partially detached, and the sucker and the excretory bladder showed granular degeneration, ang the intestine and the uterus were stained very poorly, and the deformed eggs were moderate in number.
The above experiments have demonstrated that all of the chemicals used in this experiments showed some cholonorchicidal activity, but their modes of action are fundamentally different from each other.

목차 (Table of Contents)

• 1. 緖 論
• 2. 實驗材料와 그 方法
• 3. 實驗成積
• (1) 對照群의 成積
• (2) Hexachlorophene 投與群 成積
• (3) Dithiazanine iodide 投與群 成積
• (4) Fuadin 投與群 成積
• (5) Chloroquine diphosphate 投與群 成積
• (6) Genlian violet 投與群 成積
• 4. 總括 및 考察
• 5. 結 論
• 參 考 文 獻