Application of Polymerase Chain Reaction with Disposable Amplicon Detection Device for Identification of Regulatory Gene Introduced into Genetically Modified Maize

다국어 초록 (Multilingual Abstract)

A novel genetically modified organism (GMO) detection method was developed using a simple and rapid procedure to identify a regulatory gene introduced into genetically modified (GM) maize. After DNA extraction from GM maize flours, fragments of the conserved sequence of the cauliflower masaic virus (CaMV) 35S promoter gene along with a competitive internal control gene were amplified using duplex polymerase chain reaction (PCR). Amplification products were then detected using a disposable detection device. The quantitative detection limit for GM maize 59122 was found to be 1.0% (w/w). Because the combination-method involving PCR technology coupled with a disposable detection device does not require expensive instrumentation or expertise, it will serve as a valuable alternative to immunoassays and traditional PCR-based tests in the detection of GMOs.

참고문헌 (Reference)

• 1 Yang L, "Validation of a tomato-specific gene,LAT52,used as an endogenous reference gene in qualitative and real-time quantitative PCR detection of transgenic tomatoes" 53 : 183-190, 2005
• 2 Yamaguchi H, "Two detection methods of genetically modified maize and the state of its import into Japan" 14 : 201-206, 2003
• 3 Stephenson JR, "Release of genetically modified microorganisms into the environment" 65 : 5-14, 1996
• 4 Kim HY, "Regulation and detection methods for genetically modified foods in Korea" INT UNION PURE APPLIED CHEMISTRY 82 (82): 129-137, 2010
• 5 Meyer R, "Polymerase chain reaction(PCR)in the quality and safety assurance of food:Detection of soya in processed meat products" 203 : 339-344, 1996
• 6 Allmann M, "Polymerase chain reaction(PCR):A possible alternative to immunochemical methods assuring safety and quality of food:Detection of wheat contamination in non-wheat food products" 196 : 248-251, 1993
• 7 Holst-Jensen A, "PCR technology for screening and quantification of genetically modified organisms(GMOs)" 375 : 985-993, 2003
• 8 Tang W, "Nucleic acid assay system for tier II laboratories and moderately complex clinics to detect HIV in low-resource settings" 201 : S46-S51, 2010
• 9 Michelini E, "New trends in bioanalytical tools for the detection of genetically modified organisms:An update" 392 : 355-367, 2008
• 10 Takada K, "New rapid polymerase chain reaction-immunochromatographic assay for Porphyromonas gingivalis" 76 : 508-512, 2005
• 11 Goldmeyer J, "Identification of Staphylococcus aureus and determination of methicillin resistance directly from positive blood cultures by isothermal amplification and a disposable detection device" 46 : 1534-1536, 2008
• 12 Vollenhofer, S, "Genetically modified organisms in food-screening and specific detection by polymerase chain reaction" 47 : 5038-5043, 1999
• 13 Gruère GP, "Explaining international differences in genetically modified food labeling policies" 17 : 393-408, 2009
• 14 Jae-Hwan Kim, "Event-specific Detection Methods for Genetically Modified Maize MIR604 Using Real-time PCR" 한국식품과학회 18 (18): 1118-1123, 2009
• 15 Soo PC, "Direct and simultaneous identification of Mycobacterium tuberculosis complex(MTBC)and Mycobacterium tuberculosis(MTB)by rapid multiplex nested PCR-ICT assay" 66 : 440-448, 2006
• 16 Oguchi T, "Development of multiplex PCR method for simultaneous detection of four events of genetically modified maize:DAS-59122-7,MIR604,MON863 and MON88017" 51 : 92-100, 2010
• 17 Meyer R, "Development and application of DNA analytical methods for the detection of GMOs in food" 10 : 391-399, 1999
• 18 Lin HY, "Detection of genetically modified soybeans by PCR method and immunoassay kits" 9 : 160-166, 2001
• 19 García-Cañas V, "Detection of genetically modified maize by the polymerase chain reaction and capillary gel electrophoresis with UV detection and laser-induced fluorescence" 50 : 1016-1021, 2002
• 20 Bertheau Y, "Detection methods and performance criteria for genetically modified organisms" 85 : 801-808, 2002
• 21 Permingeat HR, "Detection and quantification of transgenes in grains by multiplex and real-time PCR" 50 : 4431-4436, 2002
• 22 Engel KH, "Current and future benefits from the use of GM technology in food production" 127 : 329-336, 2002
• 23 Chandler SF, "Biotechnology in ornamental horticulture" 41 : 591-601, 2005
• 24 Chow WHA, "Application of isothermal helicase-dependent amplification with a disposable detection device in a simple sensitive stool test for toxigenic Clostridium difficile" 10 : 452-458, 2008
• 25 Forte VT, "A general multiplex-PCR assay for the general detection of genetically modified soya and maize" 16 : 535-539, 2005