HNF4α 유전자 내 SNP가 한국 재래계의 성장에 미치는 효과 분석

다국어 초록 (Multilingual Abstract)

The HNF4α (hepatocyte nuclear factor 4, alpha) is a hepatic transcription factor related to the lipid metabolism and regulation of insulin secretion in humans. The current study about commercial broiler reported that the A543G single nucleotide polymorphism (SNP) within HNF4α gene has an effect on fat deposition and wing yield in the chicken. This study was performed to investigate the association between the SNP within HNF4α gene and growth trait and to verify the applicability as a molecular marker for the improving the performance in Korean native chickens (KNCs). A total of 764 KNCs was collected from the livestock farm of Gyeongnam National University of Science and Technology in Korea and genotyped by PCR-RFLP. The body weight measured at birth, 2, 4, 6, 8, 10, 12, 14, 15, 16, 18, 20, 24, 28, 32, 36, and 40 weeks of age for the association analysis between chicken growth and the SNP genotype. Statistical analysis of the SNP with phenotypic traits was performed using SAS program. The KNC strain was classified by the genotypes of the A543G SNP. The frequencies of three genotypes were 0.47 (AA), 0.43 (AG) and 0.10 (GG), respectively. The SNP of HNF4α has highly significant association (p<0.001) with all-round growth in KNCs. These results suggest that the A543G SNP within HNF4α gene could be a genetic marker for the breeding in Korean native chickens.

목차 (Table of Contents)

• ABSTRACT
• Ⅰ. 서론
• Ⅱ. 재료 및 방법
• 1. 공시동물
• 2. Genomic DNA 추출
• 3. HNF4α 유전자의 primer 설계 및 합성
• 4. HNF4α 유전자의 PCR 증폭
• 5. PCR-RFLP 분석에 의한 HNF4α A543G SNP의 유전자형 확인
• 6. 통계분석
• Ⅲ. 결과 및 고찰
• Ⅳ. 요약
• 사사
• Ⅴ. REFERENCES

참고문헌 (Reference)

• 1 오재돈, "한국재래닭의 Uncoupling protein 유전자 Exon 3에서의 +1316T/T 유전자형이 산란율에 미치는 효과 분석" 한국가금학회 32 (32): 2005
• 2 김수원, "한국인에서의 hepatocyte nuclear factor-4α의 유전자 다형성과 제2형 당뇨병과의 연관성" 한국생명과학회 19 (19): 362-365, 2009
• 3 안동현, "한국 토종 닭고기의 정미 성분 중 유리아미노산과 핵산관련물질에 대한 연구" 한국식품영양과학회 31 (31): 547-552, 2002
• 4 Muller, Y. L., "Variants in hepatocyte nuclear factor 4α are modestly associated with type 2 diabetes in Pima Indians" 54 (54): 3035-3039, 2005
• 5 Bell, J. I., "Single nucleotide polymorphism and disease gene mapping" 4 (4): S273-S278, 2002
• 6 Wang, Z., "SNPs, Protein structure, and disease" 17 : 263-270, 2001
• 7 이지연, "OCX-32 유전자 내 c.494A>C 및 c.267T>G SNP이 한국 재래닭 산란형질에 미치는 효과 분석" 한국가금학회 41 (41): 191-196, 2014
• 8 Li, J., "Mammalian hepatocyte differentiation requires the transcription factor HNF-4alpha" 14 : 464-474, 2000
• 9 Ministry of Agriculture, Food and Rural Affairs, "Major Statistics for Agricultural Product 2016"
• 10 Sladek, F. M., "Liver-enriched transcription factor HNF-4 is a novel member of the steroid hormone receptor superfamily" 4 (4): 2353-2365, 1990
• 11 Wang, H., "Hepatocyte nuclear factor 4α regulates the expression of pancreatic β-cell genes implicated in glucose metabolism and nutrient-induced insulin secretion" 275 (275): 35953-35959, 2000
• 12 Hayhurst, G. P., "Hepatocyte nuclear factor 4alpha (nuclear receptor 2A1) is essential for maintenance of hepatic gene expression and lipid homeostasis" 21 (21): 1393-1403, 2001
• 13 Silva, V. H., "Hepatocyte nuclear factor 4 gene associated with bone traits and birth weight in male chickens" 27-, 2013
• 14 Yin, L., "Hepatic hepatocyte nuclear factor 4a is essential for maintaining triglyceride and cholesterol homeostasis" 31 (31): 328-336, 2011
• 15 H.S. Kong, "Genetic Variation and Relationships of Korean Native Chickens and Foreign Breeds Using 15 Microsatellite Markers" 아세아·태평양축산학회 19 (19): 1546-1550, 2006
• 16 Bell, G. I., "Gene for non-insulin-dependent diabetes mellitus (maturity-onset diabetes of the young subtype) is linked to DNA polymorphism on human chromosome 20q" 88 (88): 1484-1488, 1991
• 17 최준호, "Differences in the Quality Characteristics between Commercial Korean Native Chickens and Broilers" 한국축산식품학회 30 (30): 13-19, 2010
• 18 전희준, "Comparison of the Chemical Composition, Textural Characteristics, and Sensory Properties of North and South Korean Native Chickens and Commercial Broilers" 한국축산식품학회 30 (30): 171-178, 2010
• 19 김수원, "Association of Hepatocyte Nuclear Factor-4α (HNF-4α) Polymorphisms (rs1884614) with Type 2 Diabetes in Korean Population" 대한의생명과학회 15 (15): 101-103, 2009
• 20 Silva, V. H., "A SNP In the hepatocyte nuclear factor 4 gene associated with carcass traits in males from a paternal Broiler line" (suppl. 1) : 2012