어류 병원성 세균 공격 후 마늘, Allium sativum 착즙액의 침지가 넙치, Paralichthys olivaceus의 면역반응에 미치는 영향 = Effects of garlic Allium sativum extract immersion on the immune responses of olive flounder Paralichthys olivaceus prechallenged with pathogenic bacteria

다국어 초록 (Multilingual Abstract)

This study was aimed to investigate the effects in different immersion doses of garlic, Allium sativum, juice to modulate on the nonspecific immune responses of the olive flounder, Paralichthys olivaceus, artificially prechallenged with Streptococcus iniae BS10 and Edwardsiella tarda KE-1, respectively. The nonspecific immune responses of the tested fish were assessed in term of skin mucus lysozyme activity, the change of bacterial cell counts in organs, the number of lymphocytes and neutrophils in blood, and SOD activity. Almost groups of the prechallenged with either S. iniae BS10 or E. tarda KE-1 fish which had been immersed in garlic juice showed the enhanced skin mucus lysozyme activity, the number of lymphocytes and neutrophils in blood, and SOD activity in the kidney but the decreased the number of bacterial cell in surveyed organs. RPS in the group immersed in 0.25 g/L of garlic juice was much higher than in other immersed test groups. These results suggested that the garlic juice immersion can be effective on enhancement of the nonspecific immune responses and the protective ability of olive flounder to the artificial challenge with S. iniae BS10 and E. tarda KE-1.

참고문헌 (Reference)

• 1 우승호, "해수 양식 어류에서 분리된 연쇄상구균의 종류와 병원성" 한국어병학회 19 (19): 17-33, 2006
• 2 이준희, "마늘, Allium sativum이 넙치, Paralichthys olivaceus의 면역반응에 미치는 영향" 한국어병학회 23 (23): 69-83, 2010
• 3 Engstad, R.E., "Yeast glucan induce increase in activity of lysozyme and complement-mediated haemolytic activity in Atlantic salmon blood" 2 : 287-297, 1992
• 4 Raa, J., "The use of immunostimulants to increase resistance of aquatic organisms to microbial infections. in: Dis. Asian A. Vol. 1" Fish Health Section, Asian Fish. Soc. 39-50, 1992
• 5 Fu, Y.W., "The immunostimulatory effects of hot-water extract of Gelidium amansii via immersion, injection and dietary administrations on white shrimp Litopenaeus vannamei and its resistance against Vibrio alginolyticus" 25 : 673-685, 2007
• 6 Murray, C.K., "The immunohistochemical location of lysozyme in plaice (Pleuronectes platessa L.) tissue" 9 : 329-334, 1976
• 7 Miles, A.A., "The estimate of the bactericidal power of the blood" 38 : 873-885, 1938
• 8 Solem, S.T., "Stimulation of respiratory burst and phagocytic activity in Atlantic salmon Salmo salar L. macrophages by lipopolysaccharide" 5 : 475-491, 1995
• 9 Aoki, T., "Present and future problems concerning the development of drug resistance in Aquaculture, In Chemotherapy in Aquaculture : From Theory to Reality" Office International Des Epizooties 254-262, 1992
• 10 Grondel, J.L., "Pharmacokinetics and tissue distribution of oxytetracycline in carp, Cyprinus carpio L., following different routes of administration" 10 : 153-163, 1987
• 11 Jørgensen, J.B., "Peritoneal and head kidney cell response to intraperitoneally injected yeast glucan in Atlantic salmon, Salmo salar L" 16 : 313-325, 1993
• 12 Ordas, M.C., "Modulation of the chemiluminescence response of mediterranean mussel (Mytilus galloprovincialis) haemocytes" 10 : 611-622, 2000
• 13 Kodama, H., "Macrophage-colony stimulating activity in rainbow trout Oncorhynchus mykiss serum. in: Modulators of fish Immune Responses, Vol. 1" SOS Publication 59-66, 1994
• 14 Siwicki, A.K., "In vitro immunostimulation of rainbow trout Oncohynchus mykiss spleen cells with lavamisole" 14 : 231-237, 1990
• 15 Song, Y.L., "Immunostimulation of tiger shrimp (Penaeus monodon) hemocytes for generation of microbicidal substances: analysis of reactive oxygen species" 18 : 201-209, 1994
• 16 Villamil, L., "Immunomodulatory effects of nisin in turbot (Scophthalmus maximus L.)" 14 : 157-169, 2003
• 17 Kyo, E., "Immunomodulatory Effects of Aged Garlic Extract" 131 : 1075S-1079S, 2001
• 18 Kitao, T., "Immuno-stimulation of antibodyproducing cells and humoral antibody to fish bacterins by a biological response modifier" 31 : 87-91, 1987
• 19 Campa-Córdova, A.I., "Generation of superoxide anion and SOD activity in haemocytes and muscle of American white shrimp (Litopenaeus vannamei) as a response to β-glucan and sulphated polysaccharide" 12 : 353-366, 2002
• 20 Schwartz, I.F., "Garlic attenuates nitric oxide production in rat cardiac myocytes through inhibition of inducible nitric oxide synthase and the arginine transporter CAT-2 (cationic amino acid transporter-2)" 102 : 487-493, 2002
• 21 Yano, T., "Enhancement of the resistance of carp Cyprinus carpio to experimental Edwardsiella tarda infection, by some ß-1,3 glucans" 55 : 1815-1819, 1991
• 22 Kajita, Y., "Enhancement of non-specific cytotoxic activity of leucocytes in rainbow trout Oncorhynchus mykiss injected with growth hormone" 2 : 155-157, 1992
• 23 Kim, K.H., "Enhancement of chemiluminescent response of phagocytic cells from juvenile Rockfish, Sebastes schlegeli, by oral administration of levamisole" 1 : 42-47, 1998
• 24 Jeney, G., "Enhanced immune response and protection in rainbow trout to Aeromonas salmonicida bacterin following prior immersion in imunostimulants" 3 : 51-58, 1993
• 25 Kawakami, H., "Effects of iron- saturated lactoferrin on iron absorption" 52 : 903-908, 1998
• 26 Jørgensen, J.B., "Effect of a yeast-cell wall glucan on the bactericidal activity of rainbow trout macrophages" 3 : 267-277, 1993
• 27 이덕찬, "Edwardsiella tarda extracellular products (ECPs)의 인위 투여에 따른 넙치, Paralichthys olivaceus의 생체 반응" 한국어병학회 18 (18): 215-225, 2005
• 28 Yeh, S.P., "Dietary sodium alginate administration affects fingerling growth and resistance to Streptococcus sp. and iridovirus, and juvenile non-specific immune responses of the orange-spotted grouper, Epinephelus coioides" 25 : 19-27, 2008
• 29 Sakai, M., "Current research status of fish immunostimulants" 172 : 63-92, 1999
• 30 Zhang, Z., "Bath immunostimulation of rainbow trout (Oncorhynchus mykiss) fry induces enhancement of inflammatory cytokine transcripts, while repeated bath induce no changes" 26 : 677-684, 2009
• 31 Arora, D.S., "Antimicrobial activity of spices" 12 : 257-262, 1999
• 32 Benkeblia, N., "Antimicrobial activity of essential oil extracts of various onions (Allium cepa) and garlic (Allium sativum)" 37 : 263-268, 2004
• 33 Ankri, S., "Allicin from garlic strongly inhibits cysteine proteinases and cytopathic effects of Entamoeba histolytica. Antimicrob" 41 : 2286-2288, 1997
• 34 Selaraj, V., "Administration of yeast glucan enhances survival and some non-specific and specific immune parameters in carp (Cyprinus carpio) infected with Aeromonas hydrophila" 19 : 293-306, 2005
• 35 O'Gara, E.A., "Activities of garlic oil, garlic powder, and their diallyl constituents against Helicobacter pylori" 66 : 2269-2273, 2001
• 36 Reeve, V.E., "A garlic extract protects from ultraviolet B (280-320 nm) radiation-induced suppression of contact hypersensitivity" 58 : 813-817, 1993