심비디움(Cymbidium goeringii REICH B.f.) 生長点 培養에 있어서 生長調節劑, 營養 및 非營養因子가 器官形成에 미치는 影響

국문 초록 (Abstract)

난과식물에 있어서 莖頂培養은 virus 無病株生産보다는 能率이 좋은 增植方法으로 同一遺傳子組成을 갖은 苗의 多量繁植에 目的이 있으며,Cymbidium의 莖頂培養에서 protocorm을 獲得하고 또한 protocorm培養時 器官形成을 促進하고 優良苗를 育成하기 위하여 生長調節劑, 營養因子, 非營養因子別로 試驗을 實施한 結果는 다음과 같다.
莖頂培養에서 protocorm形成은 8∼10週가 소요되었으며, NAA 0.5 ppm과 BA 1.0 ppm 混合添加培地가 protocorm形成에 가장 效果的이었다.
Cymbidium protocorm으로 부터 器官分化는 NAA 0.5ppm과 BA 1.0ppm 混合添加培地에서 가장 良好하였다.
糖類는 glucose 2% 添加培地에서 器官分化가 가장 效果的이었으며, sucrose 濃度는 3% 添加培地에서 器官形成이 가장 良好하여 適正水準이었다.
窒素源은 protocorm形成에 影響이 없었으며 不定芽, 不定根形成에는 5mM의 NH_4NO₃와 NaNO₃가 效果的이었다.
Activated charcoal은 不定芽形成에 有效하였고 그適正水準은 1.0% 添加가 가장效果的이었다.
器官形成에 培地의 適正 pH는 5.8로 나타났다.
明暗處理는 器官分化 樣狀에 影響을 주었는데 不定芽形成에 24時間 光處理가 그리고 不定根이나 protocorm 形成에는 12時間이 좋은 結果를 보였다.

다국어 초록 (Multilingual Abstract)

Shoot tip culture of orchid family is mainly designed to propagate rapidly homogenious seedlings rather then the production of virus - free stoks.
In the shoot tip culture of Cymbidium (Cymbidium georingii REICH Bf) protocorm obtained was used to investigate the effect of growth regulators, nutritive and non -nutritive factors on the organogenesis.
The results obtained were as follows.
1. In the shoot tip culture, the formation of protocorm required 8∼10 weeks and MS medium supplemented with NAA 0.5ppm + BA l.0ppm was the most effective.
2. The concentration of 0.5ppm NAA and l0ppm BA showed the good organogenesis from protocorm.
3.Out of sugars tested, glucose 2% showed the best result is terms of organogenesis. In addition, the sucrose concentration of 3% appeared to be optimum
4. There was no effect of different nitrogen sources on the protocorm formation. However, the formation of adventitious shoot and root was facilitated by the addition of NH₄NO3 and NaNO₃ 5mM respectively.
5. Activated charcoal was effective in the formation of adventitious shoot with the concentration 1%.
6. Optimum pH of medium for organogenesis was 5.8.
7. Photoperiod showed the positive effects on the differentiation of organ. Results showed that 24 hours for adventitious shoot and 12 hours for protocorm was effective.

목차 (Table of Contents)

• 목차
• 抄錄 = 1
• I. 緖言 = 2
• II. 硏究史 = 3
• III. 材料 및 方法 = 7
• IV. 結果 및 考察 = 10
• 1. 生長調節劑의 影響 = 10
• 2. 營養因子의 影響 = 13
• 가. 糖類 및 濃度 = 13
• 나. Sucrose의 濃度 = 14
• 다. 窒素源 = 15
• 라. Activated charcoal = 17
• 3. 非營養因子의 影響 = 18
• 가. pH 水準 = 18
• 나. 明暗處理 = 19
• V. 摘要 = 21
• VI. 引用文獻 = 22
• Abstract = 27
• Figure Explantion = 28