행인(杏仁)이 천식관련 chemokine 분비와 호산구 chemotaxis에 미치는 영향 = Armeniacae Amarum Semen Contributes to the Chemotaxis of Eosinophils and Secretion of Chemokines in A549 Human Epithelial Cells

다국어 초록 (Multilingual Abstract)

Objective: Eosinophils are typically characterized by a bilobar nucleus with highly condensed chromatin and cytoplasm containing two major types of granules, specific and primary granules, and lipid bodies. The role of inflammation in asthma and other allergic diseases of the airways is widely appreciated, and airway inflammation is now included as a defining feature of asthma. The importance of the presence of eosinophils in the airways of patients with fetal asthma has long been recognized, but the mechanism by which these cells are recruited and retained in the lungs are only now being elucidated. Eotaxin is a potent and specific eosinophil chemoattractant that is mobilized in the respiratory epithelium after allergic stimulation. Methods : Water extracts of Armeniacae Amarum Semen(AAS) and pulmonary epithelial cell lines A549(alveolar typeII epithelial cells) and human eosinophils were used. Cytotoxic effects of AAS and MIS assay were estimated, as well as the effects of AAS on chemokines from prestimulated A549 cells by sandwich ELISA and RI-PCR. Chemotaxis assay was conducted on prestimulated eosinophils treated with AAS. Results : In this study it is demonstrated that $TGF-{\alpha}$, IL-4 and $IL-1{\beta}$ induced the accumulation of chemokine mRNAs in the alveolar epithelial cell lines A549 in dose-dependent manner. Eotaxin and IL-8 were inhibited by AAS in dose-dependent manner(p<0.05). Eosinophil migration was inhibited at high concentrations of AAS(p<0.05). Conculusions : These findings are indicative of suppression of eotaxin and IL-8, and suggest that this is accomplished through AAS treatment. This raises the possibility that AAS is of therapeutic value in diseases such as asthma.

참고문헌 (Reference)

• 1 "황기와 행인이 면역세포의 Apoptosis 및 Nitric Oxide에 미치는 효과" 6 (6): 175-186, 1998
• 2 "호산구와 천식" 46 (46): 5-16, 1999
• 3 "호산구와 관련된 chemokine에 대한 새로운 조명" 21 (21): 161-172, 2001
• 4 "행인의 알레르기 천식 효과에 대한 연구" 18 (18): 201-208, 2003
• 5 "행인반하탕이 GUINEA PIG의 기관지평활근에 미치는 영향" 13 (13): 124-34, 1992
• 6 "행인과 길경이 Asthma model 내의 Cytokine IL4 IL6에 미치는 영향" 21 (21): 31-38, 2000
• 7 "천사군자탕과 수질(마황초)가 인간 기관지상피세포의 IL6 IL1 GM CSF mRNA level에 미치는 영향" 22 (22): 604-612, 2001
• 8 "알레르기 질환에서의 유착분자" 60 (60): 568-572, 1995
• 9 "신편 중약대사전.재판" 898-900, 1985
• 10 "사람 비만細胞柱에서의 케모카인에 對한 地黃平喘湯의 效果" 23 (23): 375-385, 2002
• 11 "사람 Mast cell에서의 케모카인에 대한 白蓮治哮湯의 효과" 25 (25): 356-363, 2004
• 12 "목단피가 천식유발 cytokine 분비화 호산구 chemotaxis에 미치는 영향" 26 (26): 199-212, 2005
• 13 "동의폐계내과학" 도서출판 국진 192-199, 2004
• 14 "금은화가 천식유발 cytokine 분비화 호산구 chemotaxis에 미치는 영향" 26 (26): 129-142, 2005
• 15 "麻杏甘石湯과 桔梗이 인간기관지 상피세포의 Cytokine에 미치는 영향" 17 (17): 230-241, 2001
• 16 "麥門冬淸肺飮과 麥門冬이 인간 기관지상피세포의 IL6 IL16 GM CSF mRNA level에 미치는 영향" 23 (23): 11-23, 2002
• 17 "瀉白散과 瀉白散加沙蔘이 인간 기관지상피세포의 IL6 IL8 및 GM CSF 발현에 미치는 영향" 23 (23): 15-24, 2002
• 18 "小靑龍湯과 小靑龍湯加沙蔘이 BEAS-2B 인간 기관지상피세포의 IL6 IL8 및 GM CSF mRNA level에 미치는 영향" 24 (24): 74-83, 2003
• 19 "The role of eosinophils and neutrophils in inflammation" 30 (30): 22-27, 2000
• 20 "The Eotaxin Chemokines and CCR3 Are Fundamental Regulators of Allergen Induced Pulmonary Eosinophilia" 175 (175): 5341-5350, 2005
• 21 "RANTES- and interleukin-8-induced responses in normal human eosinophils:effects of priming with interleukin-5" 83 (83): 3697-704, 1994
• 22 "National Asthma Education and Prevention Program Expert Panel Report 2" National Institutes of Health 97 (97): 3-, 1997
• 23 "Molecular cloning of a novel human CC chemokine Eotaxin3 that is a functional ligand of CC chemokine receptor 3" 274 (274): 27975-27980, 1999
• 24 "Interleukin8 is a potent mediator of eosinophil chemotaxis through endothelium and epithelium" 268 : 117-122, 1995
• 25 "IL16 activates plasminogen plasmin system and promotes human eosinophil migration into extracellular matrix via CCR3 chemokine mediated signaling and by modulating CD4 eosinophil expression" 173 (173): 4417-4424, 2004
• 26 "ICAM1 co stimulates target cells to facilitate antigen presentation" 17 (17): 251-258, 2005
• 27 "Eosinophilia" 338 : 1592-1600, 1998
• 28 "Differences in Potency of CXC Chemokine Ligand 8 CC Chemokine Ligand 11 and C5a Induced Modulation of Integrin Function on Human Eosinophils" 175 (175): 6092-6099, 2005
• 29 "Differences in Potency of CXC Chemokine Ligand 8 CC Chemokine Ligand 11 and C5a Induced Modulation of Integrin Function on Human Eosinophils" 175 (175): 6092-6099, 2005
• 30 "Cytokine regulated accumulation of eosinophils in inflammatory disease" 59 (59): 793-805, 2004
• 31 "Cutting edge IL16 CD4 preferentially induces Th1 cell migration requirement of CCR5" 171 (171): 4965-4968, 2003
• 32 "Control of eosinophil toxicity in the lung" 4 (4): 481-486, 2005
• 33 "Bronchial epithelial cells in allergic reactions" 4 (4): 305-311, 2005