수혈예정환자에서 Dia항원을 포함한 비예기항체 선별검사의 최근 11년간 경험 = Eleven Years’ Experience with Unexpected Antibody Screening Tests Including a Dia Cell in Transfusion Candidates

국문 초록 (Abstract)

배경: 보다 효율적인 혈액 공급을 위해서 type and screen (T/S)법이 권장되고 있고, Dia 항원은한국인과 동남아시아인에서 상대적으로 높은 빈도로 나타나는 것으로 보고되고 있다. 따라서 본원에서는 모든 수혈예정환자에서 Dia 선별 혈구가 포함된 항체선별검사를 실시하고 있으며, 최근 11년간의 경험을 보고하고자 한다.
방법: 2002년 1월부터 2012년 12월까지 본원에서 수혈전 검사로 의뢰된 비예기항체 선별, 동정검사를 three-cell antigen panel (Diacell I, II, Dia; Diamed, Murten, Switzerland)와 LISS/Coombs gel card and NaCl/Enzyme card를 이용하여 검사하고분석하였다.
결과: 156,161건의 항체선별검사 결과 1.23% (1,918건)에서 양성을 보였으며, 동정된 동종 비예기항체에서 anti-E (292건)으로 가장 많았고, 다음으로 anti-E,c가 127건, anti-Lea가 103건, anti-Dia 가 91건으로 나타났다.
결론: 본원에서 시행된 비예기항체 선별검사에서 양성으로 나타났고, 동정검사를 시행하여검출된 동종비예기항체들 중에서 항-Dia 항체가4번째로 자주 나타나는 것을 알 수 있었다. 따라서 우리나라에서 보다 안전한 혈액 공급을 하기위해서는 Dia 선별 혈구가 포함된 비예기항체 선별검사를 시행해야 할 것으로 생각된다.

다국어 초록 (Multilingual Abstract)

Background:The Dia antigen has been detected with a relatively higher incidence among the Korean and Southeast Asian population. A ‘Type and Screen’ procedure is recommended for efficient transfusion, therefore, we perform antibody screening tests using antibody screening panels containing a Dia cell. The purpose of this study was to report on the experience of unexpected antibody screening test including a Dia cell in the Korean population.
Methods:We analyzed the results of antibody screening testing and identification performed during the recent 11-year period from January 2002 to December 2012. A commercially available three-cell antigen panel (Diacell I, II, Dia; DiaMed, Murten, Switzerland) was used for antibody screening. Antibodies were identified using a LISS/Coombs gel card and NaCl/Enzyme card, using the DiaMed-ID system (DiaMed, Murten, Switzerland).
Results:The frequency of unexpected antibodies was 1.23% (1,918/156,161); the most frequently detected antibodies were anti-E (292 samples), followed by anti-E,c (127 samples), anti-Lea (103 samples), and anti-Dia (91 samples).
Conclusion:Results of this study showed that the most identified unexpected antibodies were clinically significant, and, in particular, anti-Dia antibodies are detected frequently in the Korean population. Thus, unexpected antibody screening test including a Dia cell is thought to be helpful in Korea for safe transfusion.

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