RAW 264.7 cell에서 생강나무 가지 추출물의 항염증 효과 = Anti-inflammatory Effects or Lindera obtusilba Bulme Extracts in RAW 264.7 Cells

다국어 초록 (Multilingual Abstract)

Objective : This study aimed to investigate the activity according to the extraction method of Lindera obtusilba Bulme Twig (LO) in lipopolysaccharide (LPS) -induced RAW264.7 cells. Method : The methods of the two extractions included 70% ethanol and boiling in water (water extract, LOW； 70% ethanol extract, LOE). Total polyphenol, total flavonoid, EPPH and ABTS radical scavenging assays were performed to compare in vitro antioxidant effects. LOW and LOE (50, 100, and 200μg/mL) were applied to 0.1 μ g/mL LPS-induced RAW 264.7 macrophages in vitro. The level of nitric oxide (NO) and pro-inflammatory cytokines in the supernatant fraction were determined using Gress regent and the enzyme-linked immunosorbent assay (ELISA). The mitogen-activated protein kinase (МАРК) subgroups extracellular signal-regulated kinase (ERK), С-Jun N-terminal kinase (JNK), and p38 activation in extracts were detected via Western blot. Moreover, mRNA levels of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) were measured using Real-time polymerase chain reaction (qPCR).
Result : The increased NO level in the bPS-only-treated group was inhibited in a concentration―dependent manner by the treatment of two samples. Among the inflammatory cytokines, TNF-α was significantly inhibited only in the LOE (100 and 200 g/mL), but IL-1β and IL-6 were significantly inhibited in a concentration―dependent manner in all drug treatment groups. The protein expressions of MAPKs (ERK, JNK, and p―38) showed that the LOW treatment affected only p-ERK whereas the ELT treatment affected all three
factors. In particular, it was confirmed that the levels of COX-2 and iNOS mRNA were more dominant in the LOE treatment than in the LOW treatment.
Conclusions : Our findings indicate that the LOE treatment exerted a superior anti-inflammatory effect on inflammation induced by LPS than the LOW treatment in the occurrence of inflammation.