人蔘赤腐病菌의 榮養生理學的 硏究 = Studies on the nutritional physiology of Erwinia aralivorus of ginseng

다국어 초록 (Multilingual Abstract)

As a result of an elementary study for the physiological nutrition of setting up the composition of synthetic medium in Erwinia-araliavorus, the effect of hydrogen ion concentration, several kinds of carbon sources, inorganic nitrogen sources, phosphate sources, micro metals and concentration of potassium phosphate mono-basic, Potassium phosphate di-basic on the growth of this bacteria have been investigated and are summarized as follows;
1. The effects of hydrogen ion concentration on the growth of this bacteria in the basal medium, optimum hydrogen ion concentration range was 7.5.
After incubation the hydrogen ion concentration altered it's formal medium concentration. The strong acidity changed into weak acidity, the strong alkali into weak alkali.
2. The effects of several kinds of carbon sources on the growth of this bacteria, Soluble starch, Inulin and Maltose showed best results and followed by Sucrose and Mannite.
Organic acid in the carbon sources was not used effectively as nutritional elements.
After incubation hydrogen ion concentration was changed into weak acidity.
3. In the effect of nitrogen sources on the growth of the bacteria, Alanine as a organic compounds and KNO_3 as a inorganic compounds were respectively effective on the growth. L-Isoleucine and NaNO_2 were poor effect. Among this nitrogen sources, Alanine showed the best result and L-Isoleucine was appreciably poor effect.
After incubation the hydrogen Ion concentration altered it's formal medium concentration into weak acidity.
4. In the effect of phosphate sources on the growth of this bacteria, Potassium phosphate mono-basic was effective. Sodium phophate tri-basic, Ferric phosphate and Potassium phosphate di-basic showed comparatively good results but Ammonium phosphate mono basic and Calcium phosphate tri-basic proved less effective.
After incubation the hydrogen Ion concentration of this medium was altered into weak acidity.
5. As for the effects of micro metal sources on the growth of this bacteria, optimum concentration in each micro metal sources showed the following;
Ferrous sulfate 100(㎎/100ml), Zinc sulfate 0.1(㎎/100ml) Curpric sulfate (㎎/100ml). Magnesium Chloride 10(㎎/100ml) Manganese sulfate 10(㎎/100ml), Natrium Melybdae-nium(㎎/100ml), Calcium Chloride 10(㎎/100ml) and Boric acid 1(㎎/100ml).
Among these micro metal sources, ferrous sulfate, Zinc sulfate, Manesium chlorid0 and Natrium molybdaenium showed especially best results, followed by Calcium chloride, Manganese sulfate and Cupric sulfate.
The Boric acid showed poor effects. Also in the medium to which no metal sources were added the growth of this bacteria was ineffective.
After incubation the hydrogen Ion concentration generally changed into weak acid.
6. In the effect of Potassium phosphate mono basic and Potassium phosphate di-basic concentration on the growth of this bactecia, Potassium phosphate mono-basic showed the best result when concentration 0.1(g/100ml) was added in the basal medium.
No addition of phosphate to the basal medium was appreciably decreased and followed by Potasium phosphate di-basic 0.01(g/100ml).
The growth of this bacteria was much enhanced when concentration of Potassium phosphate mono basic 0.1(g/100ml) and Potassium phosphate di-basic 0.05(g/100ml) were added to the basal medium and the growth diminished when concentration of Potassium phosphate mono-basic 0.5(g/100ml) and Potassium phosphate di-basic 0.01(g/100ml) were added to the basal medium.
After incubation the hydrogen Ion concertration of medium generally changed into acidity.

목차 (Table of Contents)

• Ⅰ. 緖 言
• Ⅱ. 實驗材料 및 方法
• Ⅲ. 實驗 및 結果
• 1. 本菌의 生育과 水素 Ion 濃度의 影響
• 2. 本菌의 生育과 炭素源의 影響
• 3. 本菌의 生育과 窒素源의 影響
• 4. 本菌의 生育과 燐酸源의 影響
• 5. 本菌의 生育과 微量金屬鹽類의 影響
• 6. 本菌의 生育과 燐酸第一加量 및 燐酸 第二加量 濃度의 影響
• Ⅳ. 考 察
• Ⅴ. 結 論