植物組織培養에 있어서 生長物質의 效果에 關한 硏究 = Study on Effects of Growth Substances in Plant Tissue Cultures

다국어 초록 (Multilingual Abstract)

The object of this study is to clarify the effects of single and combination treatments of α-naphthalene acetic acid (NAA), 2,4-dichlorophenoxy acetic acid (2,4-D), indole-3-acetic acid (IAA) and kinetin in young stem cultures of Paeonia albiflora.
Basic medium was Murashige & Skoog's medium, and all media were adjusted to pH 5.8 before autoclaving. This experiments were carried out in the dark room and it was kept with temperature of 25±1℃.
In single treatment of above various growth substances, the optimum concentrations on callus initiation were NAA of 0.5mg/l,2,4-D of 0.5mg/l,IAA of 0.05mg/l and kinetin of 0.3mg/l.
In combination treatments of above various growth substances 2,4-D＋kinetin would be very efficient to clone formation, but did notinduce root differentiation. And, it's suggested that the effects of 2.4-D＋kinetin＋NAA, 2,4-D＋kinetin＋IAA and 2,4-D＋kinetin＋NAA＋IAA were same as 2,4-D＋kinetin, and NAA＋2,4-D showed clone formation efficiently.
Also, adding of NAA or 2,4-D established clone formation and root differentiation.
On the other hand, NAA＋kinetin would be effective to root differentiation. Nevertheless, excellent root differentiation and development would be established from the clones in which was trasfered from basic medium＋NAA (0.5mg/l) to basic medium.