Development of a functional hyaluronic acid modified with imidazole and dodecylamine for nanomedicine

목차 (Table of Contents)

• Ⅰ. Introduction 1
• 1.1 Nanoparticles 1
• 1.1.1 Advantages and materials 1
• 1.1.2 Stimuli-responsive nanoparticles 1
• 1.2 Hyaluronic acid 2
• 1.2.1 Properties of hyaluronic acid 2
• 1.2.2 Chemical modification of hyaluronic acid 2
• 1.3 Objectives 7
• Ⅱ. Materials and methods 9
• 2.1. Materials 9
• 2.2. Synthesis of HA-graft-imidazole-dodecylamine (HID) 9
• 2.3. Acid-base titration 10
• 2.4. Preparation of HID nanocarriers 10
• 2.5. CMC analysis 11
• 2.6. Characterization of HID nanocarriers 11
• 2.7. Preparation of DOX-loaded HID nanocarriers (DHNs) 12
• 2.8. In vitro drug release 13
• 2.9. Cell viability 13
• Ⅲ. Results and Discussion 15
• 3.1. Synthesis of HA-graft-imidazole-dodecylamine (HID) 15
• 3.2. pH profile of HID 17
• 3.3. pH-sensitivity of HID nanocarriers 19
• 3.4. pH-dependent drug release and anticancer efficacy of DHNs 25
• Ⅳ. Conclusion 31
• References 32
• 국문초록 45