조류 및 포유류 내 미토콘드리아 젖산탈수소효소 동위효소들의 분포와 역할

국문 초록 (Abstract)

조류 및 포유류에서 미토콘드리아를 분리하고 모노아민산화효소(EC 1.4.3.4, monoamine oxidase)의 활성을 측 정하여 미토콘드리아의 분리도를 확인하였다. 미토콘드리아 각 분획에서 젖산탈수소효소(EC 1.1.1.27, lactate dehydrogenase, LDH)의 발현도를 생화학적 및 면역화학적 방법으로 분석하였다. 조류에 비하여 포유류에서는 미토콘드리아 LDH의 활성이 낮은 것으로 나타났다. 따라서 미토콘드리아 LDH의 역할은 포유류보다 조류에서 중요시된다고 생각된다. 조류 및 포유류 모든 조직의 단백질양은 세포기질보다 미토콘드리아에서 적게 나타났다. 생쥐와 골든햄스터의 세포기질에서는 LDH A4, A3B, A2B2, AB3 및 B4 다섯 가지 동위효소 이외에 testis-specific LDH C4 동위효소가 정소 조직에서 나타났다. 닭의 미토콘드리아 내에서는 AB hybrid형 동위효소 한 종류가 나타났다. 포유류에서 미토콘드리아 LDH 동위효소들은 조직 별로 다르게 나타났다. 생쥐의 미토콘드리아 내에서는 LDH A4 및 testis-specific C4 동위효소가 나타났다. 미토콘드리아 testis-specific LDH C4 동위효소는 생쥐에서만 나타났다. 골든햄스터 미토콘드리아 내에서는 LDH B4 동위효소가 lactate oxidase로서 작용하였다. 조류 및 포유류의 대사조건 및 서식환경에 따라 미토콘드리아 LDH 동위효소의 역할도 구분되는 것으로 생각된다.

다국어 초록 (Multilingual Abstract)

Mitochondria were isolated from bird and mammals. The activity of monoamine oxidase (EC 1.4.3.4) was then measured to identify mitochondrial isolation. Lactate dehydrogenase (EC 1.1.1.27, lactate dehydrogenase, LDH) isozymes in mitochondrial fractions were analyzed by biochemical and immunochemical methods. The activity of mitochondrial LDH was lower in mammals than in bird. Therefore, the role of mitochondrial LDH seems to be more important in bird than in mammals. The concentration of protein in all tissues of bird and mammals was less in the mitochondria than in the cytosol. In the cytosol of mice and golden hamsters, testis-specific LDH C4 isozyme was expressed in testis in addition to the LDH A4, A3B, A2B2, AB3, and B4 isozymes. A single LDH AB hybrid isozyme was expressed in the chicken mitochondria. In mammals, mitochondrial LDH isozymes were differed according to tissues. LDH A4 and testis-specific LDH C4 isozymes were expressed in the mitochondria of mice. The mitochondrial testis-specific LDH C4 isozyme was expressed only in the mice. In the golden hamster mitochondria, the LDH B4 isozyme functioned as a lactate oxidase. As our results show, the mitochondrial LDH seemed to be playing the different role in the bird and mammals in relation with their metabolic conditions and habitats.

목차 (Table of Contents)

• 서론
• 재료 및 방법
• 결과 및 고찰
• References
• 초록

참고문헌 (Reference)

• 1 조성규, "햄스터와 소의 젖산탈수소효소에 대한 미토콘드리아 inhibitor의 영향" 한국생명과학회 15 (15): 100-105, 2005
• 2 염정주, "큰입우럭(Micropterus salmoides) 조직의 젖산탈수소효소 및 Monocarboxylate 수송체(MCT) 1, 2, 4" 한국생명과학회 22 (22): 98-109, 2012
• 3 조성규, "쏘가리(Siniperca scherzeri) 젖산탈수소효소 A4 동위효소의 정제 및 특성" 한국생명과학회 19 (19): 256-263, 2009
• 4 염정주, "기아상태에서 Ldh-C가 발현된 어류 조직의 젖산탈수소효소의 대사" 한국생명과학회 26 (26): 155-163, 2016
• 5 Schnaitman, C., "The submitochondrial localization of monoamine oxidase:an enzymatic marker for the outer membrane of rat liver mitochondria" 32 : 719-735, 1967
• 6 Passarella, S., "The mitochondrial L-lactate dehydrogenase affair" 8 : 407-, 2014
• 7 Holbrook, J. J., "The Enzymes Vol. XI" Academic Press Inc 191-192, 1975
• 8 Brooks, G. A., "Role of mitochondrial lactate dehydrogenase and lactate oxidation in the intracellular lactate shuttle" 96 : 1129-1134, 1999
• 9 Tabor, C. W., "Purification of amine oxidase from beef plasma" 208 : 645-661, 1954
• 10 Park, S. Y., "Purification and characterization of lactate dehydrogenase eye- and testis-specific C4isozyme" 15 : 263-270, 1997
• 11 Blanco, A., "Properties of the testicular lactate dehydrogenase isoenzyme" 153 : 165-172, 1976
• 12 Elustondo, P. A., "Physical and functional association of lactate dehydrogenase (LDH) with skeletal muscle mitochondria" 288 : 25309-25317, 2013
• 13 De Bari, L., "Partial reconstruction of in vitro gluconeogenesis arising from mitochondrial L-lactate uptake/metabolism and oxaloacetate export via novel L-lactate translocators" 380 : 231-242, 2004
• 14 Cho, S. K., "Mitochondrial lactate dehydrogenase in tissues of vertebrate" Cheongju Univ 2000
• 15 Passarella, S., "Mitochondria and L-lactate metabolism" 582 : 3569-3576, 2008
• 16 Schurr, A, "Lactate: the ultimate cerebral oxidative energy substrate?" 26 : 142-152, 2006
• 17 Blanco, A., "Lactate dehydrogenases in human testes" 139 : 601-602, 1963
• 18 Heinova, D., "Lactate dehydrogenase isoenzyme distribution and patterns in chicken organs" 67 : 309-312, 1999
• 19 Yasuda, J., "Lactate dehydrogenase and creatine phosphokinase isoenzymes in tissues of laboratory animals" 38 : 19-29, 1990
• 20 Goldberg, E., "LDHC: the ultimate testis-specific gene" 31 : 86-94, 2010
• 21 Baba, N., "Histochemistry of lactic dehydrogenase in heart and pectoralis muscles of rat" 51 : 621-635, 1971
• 22 Markert, C. L., "Evolution of a gene: multiple genes for LDH isozymes provide a model of the evolution of gene structure, function and regulation" 189 : 102-114, 1975
• 23 Dianzani, M. U, "Distribution of lactic acid oxidase in liver and kidney cells of normal rats and rats with fatty degeneration of the liver" 50 : 181-186, 1951
• 24 Davis, B. J, "Disc electrophoresis-Ⅱ. Method and application to human serum proteins" 121 : 404-427, 1964
• 25 Whitt, G. S, "Developmental genetics of the lactate dehydrogenase isozymes of fish" 175 : 1-35, 1970
• 26 Brooks, G. A, "Cell-cell and intracellular lactate shuttles" 587 : 5591-5600, 2009
• 27 Markert, C. L, "Biochemistry and function of lactate dehydrogenase" 2 : 131-134, 1984
• 28 Goldberg, E, "Amino acid composition and properties of crystalline lactate dehydrogenase X from mouse testes" 247 : 2044-2048, 1972
• 29 Bradford, M. M, "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding" 72 : 248-254, 1976