유도결합플라즈마질량분석기를 이용한 체내 미량금속 분석 = Trace Metal Analysis Using Inductively Coupled Plasma-Mass Spectrometry (ICP-MS)

다국어 초록 (Multilingual Abstract)

Background : The exposure of human beings to toxic trace metals continues to be an important
public health issue and concern. This study was conducted to assess the exposure to trace metals
in the general Korean population by inductively coupled plasma-mass spectrometry (ICP-MS).
Methods : Blood and urine samples were obtained from 175 healthy subjects including those who
visited the health promotion center or healthy volunteers. Specimens were collected in special containers
and we applied simple sample processing to minimize contamination. We used ICP-MS (Fisons
Instrument, Cheshire, UK) to analyze simultaneously the concentration of metals including lead, cadmium,
aluminum, cobalt, copper, zinc and selenium. The effects of age, sex and smoking on the trace
metal concentrations were also investigated.
Results : Distribution of trace metal levels in the general healthy population skewed toward lower values, which reflected low levels of exposure in the general healthy population. The concentrations of some trace metals were significantly affected by age, sex or smoking status.
Conclusions : We performed a simultaneous multielement analysis of biological samples by ICPMS, a reliable and sensitive technique, to build a database of trace metal values in the general population.
The results in this study can provide background data for future epidemiological and clinical studies associated with trace metal exposure in the Korean population. (Korean J Lab Med 2004; 24: 362-70)

국문 초록 (Abstract)

배경 : 인체의 유해미량금속에 대한 노출 정도는 중요한 공중보건학적 문제로 관심과 우려의 대상이 되고 있다. 본 연구에서는inductively coupled plasma-mass spectrometry (ICP-MS)를이용하여 혈액 및 요중 미량금속 함량을 분석하여, 한국인 일반 집단에서의 미량금속 노출 상태를 파악하고자 하였다.방법 : 건강검진 수진자 또는 건강인 자원자 175명으로부터 수집한 혈액 및 소변 검체를 연구 대상으로 하였다. 검체 수집 시에는 특수 용기를 사용하였으며 외부 오염 예방을 위해 최소한의 검체 전처리 과정을 채택하였다. ICP-MS (Fisons Instrument, Che-shire, UK)를 이용하여 혈액 및 요검체에서의 납, 카드뮴, 알루미늄, 코발트, 구리, 아연, 셀레늄 농도를 동시에 분석하였다. 또한성별, 연령 및 흡연 여부가 미량금속농도에 미치는 영향을 분석하였다.결과 : 한국인 일반 건강인에서 혈중 및 요중 미량금속 농도는낮은 농도 쪽에 치우친 분포를 보여 미량금속에의 노출 수준이 전반적으로 낮음을 시사하였다. 일부 미량금속 성분들은 혈중 또는요중 농도에 있어 성별, 연령별 또는 흡연 여부에 따른 군 간에 유의한 차이를 보였다.

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