골담초 물 추출물 및 에탄올 추출물의 이화학적 특성 및 항산화 활성 = Physicochemical Properties and Antioxidant Activity of Water and Ethanol Extracts of Caragana sinica Flower

국문 초록 (Abstract)

본 연구는 골담초 꽃 활용을 위한 연구 방법으로 온도와 시간을 달리하여 증류수로 추출하여 색도, pH, brix 및 항산화 효과를 측정하였으며, 그리고 에탄올로 추출하여 항산화 효과를 측정한 결과는 다음과 같다. 골담초 꽃 증류수 추출물의 색도는 50℃에서 1분, 5분, 10분에서 L값은 97.78~97.57 a값은 – .16~ .38, b값은 .22~1.21으로 확인되었으며, 70℃에서는 L값은 97.68~96.28, a값은 – .25~-1,23 b값은 .60~5.19로 나타났다. 그리고 100℃에서 L값은 96.57~67.92, a값은 - .63~ .57, b값은 2.17~28.89로 나타나 온도와 시간에 따라 L값은 낮아지고, a값과 b값은 높아지고 있다. pH는 50℃에서 5.53~5.18, 70℃에서는 5.40~5.25로 나타났고, 100℃에서는 4.98~4.85로 확인되어 온도가 올라갈수록 시간이 길어질수록 pH는 낮은 값이 확인되었다. brix는 50℃에서는 측정되지 않았으며, 70℃에서 5분에는 .10 brix, 10분에는 .10 brix가 측정되었으며, 100℃에서는 1분에는 .10 brix, 5분에서는 .30 brix, 10분에는 .60 brix가 측정되어 시간과 온도에 따라 brix가 변화되는 것으로 확인되었다. 골담초 꽃 증류수 추출물의 DPPH 라디칼 소거능과 ABTS 라디컬 소거능은 100℃에서 각각 42.79%, 46.34%로 가장 높은 소거능이 확인되었다. 골담초 꽃 에탄올 추출물의 500μg/mL 농도에서 DPPH 라디칼 소거능은 84.64%, ABTS 라디칼 소거능은 85.09%로 확인되었다. 총 폴리페놀 및 플라보노이드 함량은 1mg/mL 농도에서 각각 127.21μg/mL, 65.63μg/mL의 함량이 확인되었다. 이상의 연구 결과를 바탕으로 골담초 꽃은 꽃차 활용 시 저온보다는 70℃ 이상에서 추출하는 것이 효과적이며, 에탄올 추출물도 항산화 효과가 높게 확인되어 골담초 꽃은 식용과 기능성 소재로서의 가능성이 확인되었다.

다국어 초록 (Multilingual Abstract)

In this study, as a research method for utilizing Caragana sinica flowers, the color, pH, sugar content, and antioxidant effect were measured by extracting with distilled water at different temperatures and times, and theantioxidant effect was measured by extracting with ethanol. The results are as follows. The color of the distilled water extract of Caragana sinica flowers was confirmed to be 97.78~97.57 in L value, - .16~- .38 in a value, and .22~1.21 in b value at 50℃ for 1 minute, 5 minutes, and 10 minutes. At 70℃, the L value was 97.68~96.28, the a value was - .25~-1,23, and the b value was .60~5.19. And at 100℃, the L value was 96.57~67.92, the a value was - .63~ .57, and the b value was 2.17~28.89, indicating that the L value decreases and the a and b values ​​increase depending on the temperature and time. The pH was 5.53~5.18 at 50℃, 5.40~5.25 at 70℃, and 4.98~4.85 at 100℃, indicating that the pH value decreases as the temperature increases and the time increases. The sugar content was not measured at 50℃, but .10 brix was measured at 70℃ for 5 minutes and .10 brix for 10 minutes, and at 100℃, .10 brix was measured at 1 minute, .30 brix at 5 minutes, and .60 brix at 10 minutes, confirming that the sugar content changes depending on the time and temperature. The DPPH radical scavenging activity and ABTS radical scavenging activity of the distilled water extract of Caragana sinica flowers were the highest at 42.79% and 46.34%, respectively, at 100℃. The DPPH radical scavenging activity and ABTS radical scavenging activity of the ethanol extract of Gosling flower were 84.64% and 85.09%, respectively, at a concentration of 500μg/mL. The total polyphenol and flavonoid contents were 127.21μg/mL and 65.63μg/mL, respectively, at a concentration of 1mg/mL. Based on the above research results, it is effective to extract Caragana sinica flowers at 70℃ or higher rather than at low temperatures when using flower tea, and the ethanol extract was also confirmed to have a high antioxidant effect, confirming the potential of Caragana sinica flowers as an edible and functional material.