악악면 경조직 발육에 미치는 Bisphosphonate의 영향 = Effects of Bisphosphonates on Developing Hard Tissues of the Jaws in Rats

다국어 초록 (Multilingual Abstract)

Bisphosphonates are synthetic derivatives of pyrophosphonates and resistant to destruction by phrophosphatase. Bisphosphonates are currently tested for its use in orthodontic movements, osteoporosis and Paget disease treatment and prevention of metastasis in bone cancer. The effects of bisphosphonates on developing hard tissue and their action mechanism have not been elucidated yet.
This study was aimed to invetigate the effects of etidronate and pamidronate, derivatives of bisphosphonate, on hard tissue development and teeth eruption in the jaws using light and electron microscopy and TUNEL stain. The number and size of osteoclasts were also analysed suing Student's test.
Both etidronate and pamidronate inhibited teeth development and retarded their eruption. The bony trabeculae of the developing jaws in pamidronate­ treated group were thinner and more immature than those in control group. The size of osteoclasts was not significantly different between control and eitidronate and pamidronate treated groups, whereas the number of osteoclasts for the eruption of the mandibular 1st molar increased in both etidronate and pamidronte treated groups. However, many osteoclasts were detached from bone surface and did not show typical ruffled border in electron microscopic findings in both etidronate and pamidronate treated groups. No TUNEL positive osteoclasts were seen in all groups. Some of the ameloblasts and outer dental epithelial cells were abnomally proliferated and TUNEL positive in both etidronate and pamidronate treated groups.
These findings suggest that bisphosphonates can adversely delay the development of the teeth and hard tissue, so that they should be carefully used in clinics.