뱀장어(Anguilla japonica) 뇌하수체 세포의 번식 관련 유전자 mRNA 발현에 미치는 Progesterone (P4), 17β-estradiol (E2), Melatonin 및 Serotonin (5-HT)의 영향 = Effects of Progesterone (P4), 17β-estradiol (E2), Melatonin and Serotonin (5-HT) on the mRNA Expression of Reproduction-related Genes in the Pituitary Cells of Eels (Anguilla japonica)

다국어 초록 (Multilingual Abstract)

Fish reproduction is regulated by various neurohormones secreted from the brain and
gonadotropic hormones secreted from the pituitary. Reproduction of eel (Anguilla japonica)
is also regulated by these hormones. However, how the neurohormones regulate the
secretion of pituitary hormones during sexual maturation is not completely understood.
Previous studies have shown that neurohormones such as progesterone (P4), melatonin
and serotonin (5-HT) are involved in the regulation of reproductive processes in some fish.
In this study, the eel pituitary was primary cultured, and stabilized pituitary cells were
treated with P4, 17β-estradiol (E2), melatonin, or 5-HT. The effect of these treatments on
the expression of FSHβ, LHβ, GH and SL mRNA was, then, investigated. P4 increased the
expression of FSHβ and LHβ in pituitary cells, and melatonin increased the expression
of GH and SL as well as FSHβ and LHβ. However, 5-HT did not significantly affect the
expression of these mRNA. These results suggest that P4 and melatonin may play some
important roles in the early sexual maturation of eels.

국문 초록 (Abstract)

어류의 번식은 뇌에서 분비되는 다양한 신경호르몬과 뇌하수체에서 분비되는 생식소 자극 호
르몬에 의해 조절된다. 극동산 뱀장어(Anguilla japonica)의 번식도 이 호르몬들의 작용에 의해
조절되지만 성 성숙 시 신경호르몬이 뇌하수체 호르몬을 조절하는 방법은 완전히 밝혀지지 않
았다. 이전 연구에 의하면 progesterone (P4), melatonin 및 serotonin (5-HT) 등과 같은 신경호
르몬이 일부 어류의 번식 과정 조절에 관여하는 것으로 밝혀졌다. 본 연구에서는 뱀장어의 뇌
하수체를 초대 배양하였고, 안정화된 뇌하수체 세포에 P4, 17β-estradiol (E2), melatonin 및 5-
HT를 처리하였다. 이후 처리된 호르몬의 작용이 뇌하수체 세포에서 번식 관련 호르몬인 FSHβ,
LHβ, GH 및 SL mRNA 발현에 어떤 영향을 미치는지 조사하였다. 본 연구를 수행한 결과, P4는
뇌하수체 세포에서 FSHβ와 LHβ 발현을 증가시켰고, melatonin은 FSHβ와 LHβ 뿐만 아니라 GH
와 SL의 발현을 증가시켰다. 하지만 5-HT는 이 유전자의 mRNA 발현에 유의한 영향을 미치지
않았다. 이상의 결과는 P4 또는 melatonin이 뱀장어의 초기 성 성숙에 중요한 역할을 할 수
있음을 의미한다.

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