카드뮴과 구리에 노출된 풍년새우의 생태독성

국문 초록 (Abstract)

수서생태계에서 중금속 오염은 경제적, 환경보건적으로나 매우 중요한 문제이다. 최근 중금속에 의한 생태계의 안전과 건강성에 대한 우려가 대두되고, 이를 모니터링하고자 하는 시도가 이루어지는 실정이다. 이에 국내산 무갑류인 풍년새우(Branchinella kugenumaensis)를 대상으로 카드뮴(Cd)과 구리(Cu)의 장기간 노출에 따른 생물학적 반응을 파악하고자 하였다. 장기간(30일) 동안 Cd와 Cu에 노출된 풍년새우에서 생존율이 모든 농도에서 통계적 유의수준으로 급격히 감소하였다(p＜0.05). 특히 상대적으로 고농도의 Cd와 Cu 노출된 개체들이 크게 감소한 것으로 나타났고(p＜0.01), Cd 노출의 경우 농도 의존적으로 반응이 나타났다. 풍년새우의 성장률 또한 두 중금속 노출 후 모든 농도에서 감소되는 것을 확인하였다. 풍년새우의 내구란 수로 관찰한 생식율은 Cd와 Cu 노출 후 급격히 감소하는 것을 관찰할 수 있었다. 나아가, 장기적인 중금속 고농도 노출에서 풍년새우의 생식이 심각하게 저해되는 반면에, 풍년새우의 성비에는 영향이 크지 않았다. Cd 노출 후에 풍년새우에서 비대칭적인 telson 형태기형이 관찰되었으나 이러한 기형형태는 대조군에서는 전혀 발견되지 않았다. 결론적으로, 본 연구를 통해 Cd와 Cu의 중금속 장기노출에 의해 풍년새우의 생물학적 반응지표들의 변화를 확인하였다. 장기적인 노출실험 결과, 풍년새우는 중금속 노출에 대해 민감한 지표종일 뿐만 아니라 장기적인 수생태계의 모니터링을 위한 기반자료로 중요한 가치가 있을 것으로 고려되어졌다.

다국어 초록 (Multilingual Abstract)

Heavy metal contaminants on the aquatic environment are of interest because they can have severe effects on economy and public health. Recently, the studies for monitoring of heavy metals try to do on aquatic system to assess safety and health of ecosystem by heavy metals. Thus, biological responses were investigated on Korean fairy shrimp Branchinella kugenumaensis exposed to cadmium (Cd) or copper (Cu) for long-periods (30 days). The survival rate decreased significantly (p＜0.05) on B. kugenumaensis exposed to Cd and Cu at all concentrations. Especially, the highest decrease was observed at the relatively high concentration of Cd and Cu (p＜0.01) and the response by Cd exposure was at dose-dependent. The growth rates were also decreased significantly (p＜0.05) on B. kugenumaensis exposed to Cd and Cu for at all concentrations. Then, the reproduction rate, numbering cyst, was decreased significantly (p＜0.01) on B. kugenumaensis after Cd or Cu exposures. Long exposure of the relatively high concentration Cd and Cu can have severe effects on the reproduction, while exposures of Cd and Cu can not have effects on sex ratios of B. kugenumaensis. Additionally, asymmetric tel son deformity was only observed after Cd exposure. Therefore, these results suggest that B. kugenumaensis is a sensitive bio-indicator of heavy metal exposure and these biological responses of B. kugenumaensis give important information for long-term monitoring on aquatic ecosystem.

목차 (Table of Contents)

• ABSTRACT
• 서론
• 재료 및 방법
• 결과
• 고찰
• 요약
• 감사의 글
• 참고문헌

참고문헌 (Reference)

• 1 윤성명, "한국산 대형새각류(갑각 상강, 새각 강)의 분류 및 생태에 관한 연구. 한국과학재단 연구보고서" 한국과학재단 34-, 1998
• 2 Reynoldson TB, "The role of environmental factors in the ecology of tubificid oligochaetes-an experimental study" 10 : 241-248, 1987
• 3 Scott GR, "The effects of environmental pollutants on complex fish behaviour: integrating behavioural and physiological indicators of toxicity" 68 : 369-392, 2004
• 4 Yoon SM, "The Systematics and Molecular Evolution of the Korean Branchiopods (Crustacea, Branchiopoda)" Seoul National Univ. 1993
• 5 Muntau H, "Sources of cadmium, its distribution and turnover in the freshwater environment. in: Cadmium in the human environment: toxicity and carcinogenicity" International Agency for Research on Cancer 133-148, 1992
• 6 To’th L, "Some effect of CuSO4 on carp" 31 : 627-635, 1996
• 7 Bálint T, "Similarities and differences between the massive eel (Anguilla anguilla L.)devastations that occured in lake Balaton in 1991 and 1995" 37 : 17-23, 1997
• 8 Ali AJ, "Rice bran as a diet for culturing Streptocephalus proboscideus (Crustacea:Anostraca)" 486 : 249-254, 2002
• 9 USEPA, "Pollution reports on lanson chemical" U.S. Environmental Protection Agency, Emergency and Enforcement Response Branch 1992
• 10 Ishikawa C, "Phyllopod Crustacea of Japan" 7 : 154-, 1895
• 11 Rojik I, "Morphological and biochemical studies on liver, kidney and gill of fishes affected by pesticides" 34 : 81-92, 1983
• 12 USEPA, "Methods for measuring the acute toxicity of efflu-ents and receiving waters to freshwater and marine organisms. Fifth Edition" 2002
• 13 Landis WG, "Introduction to environmental toxicology: Impacts of chemicals upon ecological systems" Lewis Publ. CRC Press, Inc. 1995
• 14 Clements WH, "Integrated laboratory and field approach for assessing impacts of heavy metals at the Arkansas River, Colorado" 13 : 397-404, 1994
• 15 Massanyi P, "In vitro inhibition of the motility of bovine spermatozoa by cadmium chloride" 31 : 52-55, 1996
• 16 Brendonck L, "Feeding in the fairy shrimp Streptocephalus proboscideus (Frauenfeld) (Branchiopoda: Anostraca). II. Influence of environmental conditions on feeding rate" 13 : 245-255, 1993
• 17 Ali AJ, "Evaluation of agro-industrial wastes as diets for culture of the fairy shrimp Streptocephalus proboscideus" 298 : 167-173, 1995
• 18 Henson MC, "Endocrine disruption by cadmium, a common environmental toxicant with paradoxical effects on reproduction" 229 : 383-392, 2004
• 19 Cheng S, "Effects of heavy metals on plants and resistance mechanisms" 10 : 256-264, 2003
• 20 Maroni G, "Effects of heavy metals on Drosophila larvae and a metallothionein cDNA" 65 : 108-116, 1986
• 21 Lukac N, "Effect of cadmium on spermatozoa motility" 3 : 215-217, 2003
• 22 Maria DC, "Cyst-based toxicity tests. III. Deveolopment and standardization of an acute toxicity test with the freshwater anostracan crustacean streptocephalus proboscideus"
• 23 Schram FR, "Crustqacea" Oxford Univ. Press 1986
• 24 Skalicka M, "Copper in livestock from polluted area" 74 : 740-744, 2005
• 25 Brendonck L, "Contributions to the study of the reproductive biology of Streptocephalus proboscideus (Anostraca, Streptocephalidae)" 60 : 145-162, 1991
• 26 Dodson SI, "Cladocera and other Branchiopoda. in: Ecological and classification of North American freshwater invertebrates" Academic Press, Inc. 1991
• 27 Korenekova B, "Cadmium exposure of cattle after long-term emission from polluted area" 19 : 97-99, 2002
• 28 Eimers RD, "Cadmium accumulation in the freshwater isopod Asellus racovitzai: the relative importance of solute and particulate sources at trace concentrations" 111 : 247-258, 2001
• 29 Zar JH, "Biostatistical analysis. 2nd edition" Prentice-Hall International Inc. 718-, 1984
• 30 Landrum RF, "Bioavailability of sedimentassociated contaminants to benthic invertebrates. in: Sediments chemistry and toxicity of in-place pollutants" Lewis Publishers 237-263, 1990
• 31 Besser JM, "Bioavailability of metals in stream food webs and hazards to brook trout (Salvelinus fontinalis) in the upper Animas River watershed, Colorado" 40 : 48-59, 2001
• 32 Aoki Y, "Accumulation of cadmium and induction of its binding protein in the digestive tract of fleshfly (Sarcophaga peregrina) larvae" 77 : 279-282, 1984
• 33 Yoon SM, "A taxonomic study on the recent conchostracans of Spinicaudata (Crustacea, Branchiopoda) from Korea" 35 : 474-483, 1992
• 34 Belk D, "A confusing trio of Branchinecta (Crustacea: Anostraca) from the western North America with a description of a new species" 486 : 49-55, 2002