우 난포란(卵胞卵)의 체외성숙(體外成熟)에 관한 연구 : II. 발정우혈청(發精牛血淸) , 우난포액(牛卵胞液) 및 난구세포(卵丘細胞)가 난포란의 체외성숙과 체외수정(體外受精)에 미치는 영향 II. Effects of Estrus Bovine Serum , Bovine Follicular Fluid and Matured Cumulus Cells on In Vitro Maturation and Fertilization of Follicular Oocytes = Studies on In Vitro Maturation of Bovine Follicular Oocytes

국문 초록 (Abstract)

本 硏究는 發精牛血淸, 牛卵胞液 및 成熟卵丘細胞가 牛卵胞卵의 體外成熟과 體外受精에 미치는 效果를 調査하였으며 그 結果는 다음과 같다.
1. 發精牛血淸 50%와 成熟卵丘細胞를 添加한 m-TALP 培養液에 있어서의 卵胞卵의 成熟率은 63.7%로서 이는 基礎培養液 및 100% 發精牛血淸에 있어서의 成熟率인 40.2% 및 47.3%보다 有意하게(P<0.05) 높았다.
2. m-TALP 培養液에 牛卵胞液 50%와 成熟卵丘細胞을 添加했을 때의 卵胞卵의 成熟率은 70.5%로서 이는 基礎培養液에 있어서의 38.3%나, 100% 牛卵胞液에 있어서의 50.7%보다 有意하게(P<0.05) 높았다.
3. 發精牛血淸이나 牛卵胞液이 添加된 m-TALP 培養液에서 成熟卵丘細胞와 함께 同伴培養에 의해 成熟된 卵胞卵의 體外受精率은 22.3∼43.2%였는데 이 成績은 基礎培養液의 11.6∼17.2%보다 有意하게(P<0.01) 높았으며, 특히 25% 發精牛血淸과 25% 牛卵胞液을 添加한 培養液에서 成熟된 卵胞卵의 體外受精率은 43.2%로서 가장 높았다.

다국어 초록 (Multilingual Abstract)

These experiments were carried out to investigate the effects of estrus bovine serum(EBS), bovine follicular fluid(BFF) and matured cumulus cells(MCC) on in vitro maturation and fertilization of bovine follicular oocytes.
The ovaries and testes were obtained from slaughtered Holstein cow and bulls respectively. The follicular oocytes surrounded with cumulus cells were recovered by aspirating the follicular fluid from the visible follicles of diameter 2-6 ㎜ and the semen were prepared from bull's epididymal cauda. The follicular oocytes were cultured in m-TALP medium containing EBS, BFF or/and MCC for 32h in a incubator with 5% CO_2 in air at 38℃, and then matured oocytes were again cultured for 24h, with 5×10^5/㎖ motile sperm capacitated in the BO medium containing 5㎎/㎖ BSA and 2.5㎜ caffein.
The results obtained in these experiments were summarized as follow:
1. The maturation rate of the follicular oocytes, 63.7%, obtained from the m-TALP medium supplemented with 50% EBS and MCC was significantly higher (P<0.05) than that from basic medium (40.2%) or from 100% EBS medium (47.3%).
2. The maturation rate of the follicular oocytes cultured in m-TALP medium supplemented with 50% BFF and MCC was 70.5%. This value was significantly higher (P<0.05) than 38.3% of basic medium or 50.7% of 100% BFF medium.
3. The fertilization rates of the follicular oocytes matured in the m-TALP medium supplemented with EBS, BFF or/and MCC were ranged from 22.3% to 43.2%, and those values were significantly higher (P<0.01) than that of the follicular oocytes matured in basic medium. The highest fertilization rate, 43.2%, was obtained from m-TALP medium supplemented with EBS and BFF by 25% respectively.