에스트로겐이 T 세포 면역반응에 미치는 효과 = Effects of estrogen on the murine T cell functions

다국어 초록 (Multilingual Abstract)

Many autoimmune diseases, such as multiple sclerosis, rheumatoid arthritis, systemic lupus erythematosus and thyroiditis are more prevalent in female than male. However, the precise reasons for this gender bias are unclear, although it had been reported that Th1/Th2 immune reaction could be modulated by estrogen Therefore, this study was performed to document how CD4^+ T cell-mediated immune function are regulated by estrogen.
The CD4^+T cells were purified from male and female C57BL/6 mice and activated with anti-CD3 antibody. The proliferation rate and activation-induced cell death of CD4^+T cells from male spleen were similar to those of CD4^+T T cells from female spleen and were not affected by exogenous E2. In contrast, IFN-γ secretion from activated T cells were lower and IL-4 secretion was higher in female than male mice, IFN-γ secretion from activated T cells was also suppressed by exogenous E2, while T-4 secretion was enhanced. These results suggest that E2 shifts cytokine production from CD4' T cells toward Th2 response, and this possibility were supported by the suppression of Th1-dependent immune reaction in vitro, such as mixed lymphocyte reaction, by E2. In addition, Th1-dependent immune reaction in vivo, such as IFN-γ secretion from KLH-specific CD4^+T cells and production of anti-KLH IgG2a antibodies were suppressed in female mice, compared to male, Taken together, these results suggest that E2 regulate T cell-dependent immune reaction by modulation of cytokine production from CD4^+T cells toward Th2 dominant reaction.

목차 (Table of Contents)

• 목차
• I. 참고문헌 = 1
• II. 재료 및 방법 = 6
• 1. 연구재료 = 6
• 1) 마우스 = 6
• 2) 시약 및 항체 = 6
• 2. 연구방법 = 6
• 1) CD4- 양성 T 세포의 분리 및 배양 = 5
• 2) 면역형광염색 및 유세포측정 = 5
• 3) 에스토로겐 투여 = 5
• 4) T 세포의 활성화 및 증식능 측정 = 6
• 5) in vitro AICD = 6
• 6) Cytokine ELISA = 6
• 7) in vitro MLR = 7
• 8) KLH 면역 후 KLH 특이 T 세포 반응 = 7
• 9) KLH-특이 항체 형성능 = 8
• III. 결과 = 9
• 1. CD4^+T 세포의 증식능에 미치는 암수 차이 및 에스토로겐의 영향 = 9
• 2. CD4^+T 세포의 AICD에 미치는 에스토로겐의 영향 = 13
• 3. CD4^+T 세포 cytokine 분비능에 미치는 암수 차이 및 에스토로겐의 영향 = 15
• 4. MLR에 미치는 암수 차이 및 에스토로겐의 영향 = 18
• 5. 암수 C57BL/6 마우스에서 항-KLH CD4^+T 세포 반응 비교 = 18
• 6. 암수 C57BL/6 마우스에서 항-KLH 항체 생성능 비교 = 22
• IV. 고찰 = 24
• V. 결론 = 30
• 참고문헌 = 31