澜仕口服液对肾阳虚小鼠模型的调节作用研究 = Effect on Lanshi Tonic in Mice with Kidney-Yang Deficiency

다국어 초록 (Multilingual Abstract)

Purpose: To study the regulatory effect of Lanshi tonic on kidney-yang deficiency model and its related mechanism. Methods: ICR mice were randomly divided into five groups according to body weight: normal group, model group, Lanshi tonic high, medium and low dose group. Mice with kidney-yang deficiency syndrome were induced by hydrocortisone sodium succinate by intraperitoneal injection, 50 mg/ kg for 7 days. At the same time, all groups were given corresponding medicine by oral administration for 10 days. The body mass, hair color and body shape of mice were observed and recorded. Spleen index, thymus index and adrenal index were calculated. Level of urine 17-OHCS was measured by ELISA. Level of serum Cortisol, ACTH, cAMP, cGMP and ratio of cAMP/cGMP were measured by ELISA. Results: Compared with the normal group, the above indexes of the model group mice were significantly lower (p<0.05, p<0.01), except the serum cGMP was significantly higher (p<0.01). Compared with the model group, Lanshi tonic at high and medium doses had no significant change in urine 17-OHCS content (p>0.05), but had different improvement effects on the other indicators mentioned above (p<0.05, p<0.01).
Conclusion: Lanshi tonic can regulate the state of kidney-yang deficiency, which may be related to the regulation of adrenocortical secretion function and the imbalance of cyclic nucleotide levels.

다국어 초록 (Multilingual Abstract)

目的: 研究澜仕口服液对肾阳虚状态的调节作用及相关机制。方法: 将ICR小鼠按体质量随机分为5组：正常组、 模型组、澜仕口服液高、中、低剂量组。小鼠腹腔注射氢化可的松琥珀酸钠，50 mg/kg，连续7天，停止注射3 天造成肾阳虚证候小鼠模型。造模同时给予各剂量澜仕口服液，正常组和模型组在同等条件下给予蒸馏水。观 察并记录小鼠体质量、毛色、体态等一般体征状态，解剖、称量并计算脾指数、胸腺指数和肾上腺指数，ELISA 法检测尿液17-OHCS含量，ELISA法检测血清Cortisol、ACTH、cAMP、cGMP含量、计算cAMP/cGMP比值。结 果: 与正常组比较，模型组小鼠除血清cGMP显著升高外，上述其他指标均显著降低（p<0.05, p<0.01）。与模 型组比较，澜仕口服液高剂量和中剂量除对尿液17-OHCS含量无显著改变（p>0.05）外，对上述其他指标均有 不同程度的改善作用（p<0.05, p<0.01）。结论: 澜仕口服液对肾阳虚状态具有调节作用，可能与调节肾上腺皮 质分泌功能和调整环核苷酸水平失衡相关。

참고문헌 (Reference)

• 1 Zhang QY, "The relationship between the determination of sex hormone and syndrome differentiation in patients with chronic renal failure" 287 : 30-31, 1989
• 2 Xu HF, "The effect on immunological function of rat modle with kidney-yang deficiency syndrome of ethanol extracts of Cuscuta chinensis Lam" 38 : 2163-2165, 2015
• 3 Shen ZY, "Study on the location of kidney-yang deficiency syndrome" 17 : 50-52, 1997
• 4 Lin ZJ, "Research on animal model evaluation with traditional Chinese medicine principles" 28 : 2217-2221, 2013
• 5 Chen XY, "Practical animal model of TCM syndrome" Peking Union Medical 108-, 1993
• 6 Du J, "Methods for preparing kidney deficient models and related evaluating factors" 14 : 9433-9436, 2010
• 7 Ren YS, "Investigation on cold/heat characters of kidney yin/yang deficiency models by zoogenic thermal activity" 16 : 94-101, 2010
• 8 Gou XJ, "Investigation of model-shaping method for deficiency syndrome of kidney-yang" 29 : 814-815, 2009
• 9 Dai B, "Establishment of a mouse model of kidney deficiency induced by oral administration of hydrocortisone and evaluation of related factors" 25 : 70-73, 2017
• 10 Zhang ZY, "Establishment and stability observation of rat model of kidney-yang deficiency and kidney-yin deficiency" 46 : 51-54, 2015
• 11 Zhang D, "Establishment and evaluation in mice model with kidney-yang deficiency syndrome caused by overfating and sexual overstrain" 25 : 9-11, 2008
• 12 Qin WY, "Comparison of the different model establishment methods of kidney yang deficiency in mice caused by hydro-cortisone" 34 : 11-14, 2017
• 13 Jia KJ, "Comparasion of antis egg-case before and after preparing in reproducing kidney-yang deficiency model rats" 39 : 1516-1520, 2016