해양생물 중 마비성패독 검출을 위한 발광성 박테리아 Aliivibrio fischeri의 적용 연구 = Development of a Paralytic Shellfish Poisoning Detection Method Using Bioluminescent Bacterium, Aliivibrio fischeri

국문 초록 (Abstract)

마비성패독에 의한 중독(Paralytic shellfish poisoning, PSP)은 와편모조류 등이 생성하는 독소에 노출된 수산물에 의해 발생한다. 대표적인 마비성패독 삭시톡신(Saxitoxin, STX)은 30개 이상의 이성질체가 있으며, 종류에 따라 독성에 차이가 있고 체내에서의 독성 성분의 변화를 일으키기도 한다. 본 연구는 발광박테리아 알리비브리오(Aliivibrio fischeri)를 활용해 STX군 독소들을 스크리닝하고 발광저해율에 따른 독성 차이를 확인하였다. 이 독성평가방법을 Bioluminescence inhibition assay (BIA)라고 하며, 발광박테리아의 발광저해율로 독성 유무를 확인한다. 실험은 STX 과 9종의 이성질체(STX, GTX1&4, GTX2&3, NEO, dcNEO, dcSTX, dcGTX2&3, C1&C2, GTX5, GTX6)의 표준독소로 BIA를 진행하였고, 독성지수로 EC50 (mg/L)값을 산출하였다. 산출된 EC50값은 STX (20.9 mg/L)을 기준으로 STX group의 독성을 독성등가계수(Toxic Equivalency Factor, TEF)로 치환하였다. BIA 결과로 GTX6가 가장높은 독성을 GTX2&3이 가장 낮은 독성을 나타내었는데, 이는 WHO (2016), EFSA (2009)에서 제시한 TEF값과 상이한 것으로 나타났다. BIA를 이용한 독성평가 결과, 독소별 독성의 차이는 나타났지만 마비성패독에 대한 기질 특이성은 알려지지 않았다. BIA를MBA 대체시험법으로 제시하기 위해서는 마비성패류독소에 작용에 대한 특이성을 확인할 수 있는 연구가 필요할 것으로 사료된다.

다국어 초록 (Multilingual Abstract)

Paralytic shellfish poisoning (PSP) occurs when seafood contaminated with toxin-producing dinoflagellates (e.g. Alexandrium species, Gymnodinium species and Pyrodinium bahamense) is consumed. Saxitoxin (STX). Paralytic shellfish toxin has more Copyright © 2024 The Korean Society of Phycology This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
수생생물Aquatic Nature 2024 June, 4(1): 1-9 pISSN 2765-7876 eISSN 2799-3000 https://doi.org/10.23135/an.2024.4.1.1 2 ∙ 수생생물 제4권 제1호 (2024) 김영진 외than 30 analogues, and its toxicity varies among the causative organisms. This study evaluated the differences in toxicity of STX and nine analogues (GTX1&4, GTX2&3, NEO, dcNEO, dcSTX, dcGTX2&3, C1&C2, GTX5, GTX6) using the Bioluminescence Inhibition Assay (BIA), and the toxicity test measured the inhibition rate of luminescence of bacterium, Aliivibrio fischeri. The toxicity index was calculated as EC50 (mg/L) values. The derived EC50 values were converted to Toxic Equivalency Factors (TEF) for the STX group, using STX (20.9 mg/L) as the reference. The BIA revealed that GTX6 exhibited the highest toxicity but the toxicity of GTX2&3 was the lowest. This result differed from the TEF values suggested by WHO (2016) and EFSA (2009), indicating that BIA may lack substrate specificity for STX group. Therefore, to propose an alternative screening method to the mouse bioassay, further research is needed to confirm the specificity of BIA for paralytic shellfish toxins.

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