SCIE
SCOPUS
KCI등재
급성 및 만성 영양장애가 소화효소 활성 및 세포기능에 미치는 실험적 연구 = Dietary Induced Depletion and Repletion of Pancreatic enzymes in the Rat with Emphasis on Korean Dishes
저자
김윤수 ; 황석귀 ; 김명원 ; 유용운 ; 송정석 ( Yoon Soo Kim ; Suk Kuy Whang ; Myung Won Kim ; Yong Wun Ryu ; Chung Suk Song )
발행기관
생화학분자생물학회(Korean Society for Biochemistry and Molecular Biology)
학술지명
권호사항
발행연도
1974
작성언어
-KDC
400
등재정보
SCIE,SCOPUS,KCI등재
자료형태
학술저널
발행기관 URL
수록면
13-29(17쪽)
제공처
A variety of digestive Enzymes of pancreatic and intestinal origin participate in the breakdown of protein, carbohydrate and lipids. It has been known that some of enzyme output of the intestinal glands can be manipulated by altering components of the diet. The incidence of patients who are suffering from gastrointestinal trouble in Korea is much higher than that of the United States. This fact might correlate with diet composition of Korean and American dishes. These facts are attractive for us to study the effect of diet composition on the digestive enzymes such as amylase, lipase, trypsin and chymotrypsin, and to decide whether the difference of diet composition affects these enzymes and whether such high incidence of gastroin testinal patients in Korea could be adequately explained by change in digestive enzyme activities of the animals fed with different diet. Our previous experiments have shown that pancreatic enzyme activites of rats fed with 5% casein diet which was prepared similarly to Korean diet in terms of protein content only were depressed as much as one fifth of that of control during two weeks feeding period. but it is true that protein is not only depleted but fat is also depleted in Korean dishes. The primary objective of the experiments described herein was to study the effect on the pancreatic enzyme activities of diet which was similarly prepared to Korean dishes and also to study the effect on the pancreatic enzyme activities of varying the proportion of fat to carbohydrate in the diet which is keeping the diet isonitrogenous and isocaloric. Data from this experiment will be discussed ire conjunction with previously published results conducted in our laboratory in order to illustrate how the high incidence of gastrointestinal patients in Korea could be adequately explained by diet prepared in similar composition to the Korean dishes. In a random distribution, male albino rats, weighing the average of 80 gm each were divided into five groups. Group I ; 5% casein : no fat : carbohydrate Group II ; 5% casein : 12% fat : carbohydrate Group III ; 18% casein : no fat : carbohydrate Group IV ; 18% casein : 12% fat : carbohydrate Group V ; 18% casein : high fat : carbohydrate (ratio of fat to carbohydrate 94 : 6) Calorie difference from protein or fat depletion was substitiuted by carbohydrate. At the end of designated time interval, the rats were sacrificed 14-15 hours after the last meal by decapitation. The pancreas was carefully romoved and placed in a cold 0. 25M sucrose solution (4℃). A 5% homogenate in 0.25M sucrose was prepared from the glandular {free of obvious lymph nodes and adipose tissue) portion of the pancreas. 5:% Homogenates were centrifuged (14,000 x g) for 15 minutes to remove cellular debris and whole cells. Enzyme analysis and protein determination were subsequently performed on the supernatant. Protein was determined by Lawry method (1951). Enzyme activity assays were performed using modification of Hepler method (1962) for amylase and the method developed by Cherry and Crandall (1932) for lipase. Chymotryptic assays were performed using a modification of method developed by Rhodes et al. (1957), as a substrate for chymotrypsinogen N-acetyl-L-tyrosine ethyl ester was used. Trypsinogen assay was similarly performed using p-toluene-sulfonyl-L-arginine methyl ester as a substrate. The assays were linear with respect to enzyme concentration over the range used under specified conditions such as temperature (37℃) and incubation period, 30 minutes for amylase, 1 hour for lipase, and for the trypsinogen and chymotrypsinogen the recording of dye disappearing of optical density was recorded 2 to 3 seconds after adding substrate-buffer-indicator The results of the experiments in which the effect of different composition of nutrient which was prepared in similar composition to Korean dishes on pancreatic enzyme acitivities are summarized as the following. When the enzyme activity
더보기서지정보 내보내기(Export)
닫기소장기관 정보
닫기권호소장정보
닫기오류접수
닫기오류 접수 확인
닫기음성서비스 신청
닫기음성서비스 신청 확인
닫기이용약관
닫기학술연구정보서비스 이용약관 (2017년 1월 1일 ~ 현재 적용)
학술연구정보서비스(이하 RISS)는 정보주체의 자유와 권리 보호를 위해 「개인정보 보호법」 및 관계 법령이 정한 바를 준수하여, 적법하게 개인정보를 처리하고 안전하게 관리하고 있습니다. 이에 「개인정보 보호법」 제30조에 따라 정보주체에게 개인정보 처리에 관한 절차 및 기준을 안내하고, 이와 관련한 고충을 신속하고 원활하게 처리할 수 있도록 하기 위하여 다음과 같이 개인정보 처리방침을 수립·공개합니다.
주요 개인정보 처리 표시(라벨링)
목 차
3년
또는 회원탈퇴시까지5년
(「전자상거래 등에서의 소비자보호에 관한3년
(「전자상거래 등에서의 소비자보호에 관한2년
이상(개인정보보호위원회 : 개인정보의 안전성 확보조치 기준)개인정보파일의 명칭 | 운영근거 / 처리목적 | 개인정보파일에 기록되는 개인정보의 항목 | 보유기간 | |
---|---|---|---|---|
학술연구정보서비스 이용자 가입정보 파일 | 한국교육학술정보원법 | 필수 | ID, 비밀번호, 성명, 생년월일, 신분(직업구분), 이메일, 소속분야, 웹진메일 수신동의 여부 | 3년 또는 탈퇴시 |
선택 | 소속기관명, 소속도서관명, 학과/부서명, 학번/직원번호, 휴대전화, 주소 |
구분 | 담당자 | 연락처 |
---|---|---|
KERIS 개인정보 보호책임자 | 정보보호본부 김태우 | - 이메일 : lsy@keris.or.kr - 전화번호 : 053-714-0439 - 팩스번호 : 053-714-0195 |
KERIS 개인정보 보호담당자 | 개인정보보호부 이상엽 | |
RISS 개인정보 보호책임자 | 대학학술본부 장금연 | - 이메일 : giltizen@keris.or.kr - 전화번호 : 053-714-0149 - 팩스번호 : 053-714-0194 |
RISS 개인정보 보호담당자 | 학술진흥부 길원진 |
자동로그아웃 안내
닫기인증오류 안내
닫기귀하께서는 휴면계정 전환 후 1년동안 회원정보 수집 및 이용에 대한
재동의를 하지 않으신 관계로 개인정보가 삭제되었습니다.
(참조 : RISS 이용약관 및 개인정보처리방침)
신규회원으로 가입하여 이용 부탁 드리며, 추가 문의는 고객센터로 연락 바랍니다.
- 기존 아이디 재사용 불가
휴면계정 안내
RISS는 [표준개인정보 보호지침]에 따라 2년을 주기로 개인정보 수집·이용에 관하여 (재)동의를 받고 있으며, (재)동의를 하지 않을 경우, 휴면계정으로 전환됩니다.
(※ 휴면계정은 원문이용 및 복사/대출 서비스를 이용할 수 없습니다.)
휴면계정으로 전환된 후 1년간 회원정보 수집·이용에 대한 재동의를 하지 않을 경우, RISS에서 자동탈퇴 및 개인정보가 삭제처리 됩니다.
고객센터 1599-3122
ARS번호+1번(회원가입 및 정보수정)