제주도 한우와 제주흑우실용화축군 집단에서 ADD1 유전자의 다형성이 경제형질에 미치는 영향

국문 초록 (Abstract)

한우와 제주흑우실용화축군(JBC-DC) 집단에서 ADD1 유전자의 유전적 다형성을 조사하였다. ADD1 유전자형은 intron 7 영역에서 84-bp 절편의 유무에 따라 결정하고, 두 집단에서 ADD1 유전자형과 경제형질의 상관관계를 시험하였다. 한우 거세우집단에서는 ADD1 D/- 도체들이 WW 개체들보다 유의적으로 더 두꺼운 등지방두께를 보여주었다(p<0.05). 하지만, JBC-DC 집단에서는 이형접합인 WD에서 가장 두꺼운 등지방두께를 나타냄을 볼 수 있었고(p<0.05), 이는 W와 D 대립인자의 상승효과에 의해 등지방 축적이 증가하는 것으로 추정된다. 반면 육질등급지수나 근내지방도으로 측정된 근육조직에서 근내지방의 축적과 ADD1 유전자형과은 상관없는 것으로 조사되었다. 이상의 결과들로부터, ADD1 유전자는 근육조직의 근내지방보다는 피하조직에서 등지방에 영향을 주는 것으로 보인다. 또한 DD 유전자형에서 W/-인 동물들에 비해 더 높은 수준의 육색을 나타내었다(p<0.05). 흥미로운 것은 JBC-DC 집단에서만 도체중과 유전자형 사이에 고도의 유의적인 차이를 나타내었는데, D/-인 동물들이 WW인 동물들에 비해 38 kg 이상 더 무거웠다(p<0.001). 연구결과는 ADD1 유전자형에 따라 빠른 성장률과 거세우의 생산성에 차이들을 나타내는 것을 보여주고 있다. 이러한 발견들은 ADD1 유전자형이 유전적 분자 마커로써 한우와 제주흑우-유래 축군의 개량을 위한 교배육종에서 효과적인 역할을 수행할 수 있음을 보여주는 결과이다.

다국어 초록 (Multilingual Abstract)

Genetic polymorphisms of adipocyte determination and differentiation factor 1 (ADD1) gene were screened in Hanwoo and Jeju Black cattle-derived commercial (JBC-DC) populations. The ADD1 genotypes were determined using the presence/absence of 84-bp fragment at intron 7 region. The association of ADD1 genotypes for economic traits was examined in both populations. In the Hanwoo steers, ADD1 D/-carcasses showed significantly thicker backfat levels than those from WW (p<0.05). However, the thickest level of backfat appeared in WD heterozygotes, whereas thicker backfat did not appear in DD homozygotes in the JBC-DC population (p<0.05), leading to the supposition that synergic effects of alleles W and D increase backfat deposition. On the other hand, there was no association between the ADD1 genotypes and intramuscular fat deposition measured as meat quality index and marbling score. From these results, we concluded that the bovine ADD1 affected the backfat in subcutaneous tissue, rather than intramuscular fat in muscle tissue. In addition, the DD animals showed higher levels of meat color than those from W/- (p<0.05). Interestingly, a highly significant difference was found between the genotypes and carcass weights only in the JBC-DC population, and D/- animals were heavier by more than 38 kg than those from WW (p<0.001). The results of this study reveal faster growth rate and differences in steer productivity according to genotypes of the ADD1 gene. These findings demonstrate that ADD1 genotypes may effectively function as molecular genetic markers for the improvement of Hanwoo and Jeju Black cattle-related crossbreeding systems.

목차 (Table of Contents)

• 서론
• 재료 및 방법
• 결과 및 고찰
• References

참고문헌 (Reference)

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