CPP-ACP 적용이 치면열구전색제의 결합강도에 미치는 영향 = The effect of CPP-ACP pre-treatment on bond strength of a sealant

국문 초록 (Abstract)

최근 CPP-ACP의 초기 우식증의 재광화 및 예방 효과에 대한 연구가 많이 진행되고 있다. 그러나, CPP-ACP 적용으로 인한 법랑질 산부식 저항성 증가가 치면열구전색제 접착에 미치는 영향에 대해서는 연구가 부족한 실정이다. 본 연구에서는 CPP-ACP의 치면 도포 여부와 치면열구전색제의 접착 방법의 차이가 법랑질과 치면열구전색제의 결합력에 미치는 영향에 대하여 미세전단결합강도 측정법으로 알아보고자 하였다. 건전한 제3대구치를 사용하여 150개의 시편을 제작하였으며, 다음과 같이 6군으로 나누었다. I군: 인공타액에 2주 보관 후 35% 인산 산부식 II군: 인공타액에 2주 보관 후 35% 인산 산부식 + 접착제 III군: 인공타액에 2주 보관 후 자가 부식 접착제 IV군: CPP-ACP 2주 적용 후 35% 인산 산부식 V군: CPP-ACP 2주 적용 후 35% 인산 산부식 + 접착제 VI군: CPP-ACP 2주 적용 후 자가 부식 접착제 6군의 시편에 치면열구전색제 접착 후 미세전단결합강도를 측정하여 다음과 같은 결과를 얻었다. CPP-ACP 적용은 전통적인 산부식 방법으로 치면열구전색제를 접착하는 경우에는 결합강도와 산부식 양상에 영향을 미치 지 않으나, 자가 부식 접착제를 사용한 경우에는 치면열구전색제의 결합강도를 약화시켰다.

다국어 초록 (Multilingual Abstract)

Recently the effect of CPP-ACP on early caries remineralization and prevention has hashbeen extensively researched. However, there has been a lack of research on micro-shear bond strength of a sealant applied on a CPP-ACP treated surface. Therefore, the purpose of this study was to evaluate the effect of CPP-ACP on the micro-shear bond strength of a sealant bonded with 3 different bonding systems. From the sound human 3rd molars, the 150 specimens were prepared and randomly assigned to six groups. Group I: Immersion in artificial saliva for 2 weeks + 35% phosphoric acid Group II: Immersion in artificial saliva for 2 weeks + 35% phosphoric acid + dentin bonding agent Group III: Immersion in artificial saliva for 2 weeks + self-etching adhesive Group IV: CPP-ACP application for 2 weeks + 35% phosphoric acid Group V: CPP-ACP application for 2 weeks + 35% phosphoric acid + dentin bonding agent Group VI: CPP-ACP application for 2 weeks + self-etching adhesive Sealant was applied and the micro-shear bond strength was measured. From the result of this study, it can be assumed that the CPP-ACP pretreatment can weaken the bond strength of a sealant if the enamel surface is conditioned with self-etch adhesive.

참고문헌 (Reference)

• 1 최한주, "탈회된 법랑질에서 CPP-ACP의 재광화 깊이" 대한소아치과학회 35 (35): 278-286, 2008
• 2 강지선, "법랑질 인공우식병소에서 APF gel과 CPP-ACP 제제의 재광화 효과" 대한소아치과학회 35 (35): 684-691, 2008
• 3 Hitt JC, "Use of a bonding agent to reduce sealant sensitivity to moisture contamination: an in vitro study" 14 : 41-46, 1992
• 4 Venker DJ, "Twelve-month sealant retention in a school-based program using a self-etching primer/adhesive" 64 : 191-197, 2004
• 5 Hadavi F, "The effect of dentin primer on the shear bond strength between composite resin and enamel" 18 : 61-65, 1993
• 6 Featherstone JD, "The continuum of dental cariesevidence for a dynamic disease process" 83 (83): C39-42, 2004
• 7 Kanemura N, "Tensile bond strength to and SEM evaluation of ground and intact enamel surfaces" 27 : 523-530, 1999
• 8 Placido E, "Shear versus micro-shear bond strength test: a finite element stress analysis" 23 : 1086-1092, 2007
• 9 Feigal RJ, "Self-etch adhesives for sealants?" 19 : 69-70, 2007
• 10 Moule CA, "Resin bonding using an all-etch or self-etch adhesive to enamel after carbamide peroxide and/or CPP-ACP treatment" 52 : 133-137, 2007
• 11 Shen P, "Remineralization of enamel subsurface lesions by sugar-free chewing gum containing casein phosphopeptide-amorphous calcium phosphate" 80 : 2066-2070, 2001
• 12 Ahovuo-Saloranta A, "Pit and fissure sealants for preventing dental decay in the permanent teeth of children and adolescents" CD001830 : 2008
• 13 Cross KJ, "Physicochemical characterization of casein phosphopeptide-amorphous calcium phosphate nanocomplexes" 280 : 15362-15369, 2005
• 14 Pitts NB, "Modern concepts of caries measurement" 83 (83): C43-47, 2004
• 15 Perdigao J, "Microtensile adhesion of sealants to intact enamel" 15 : 342-348, 2005
• 16 Weerasinghe DS, "Micro-shear bond strength and morphological analysis of a self-etching primer adhesive system to fluorosed enamel" 33 : 419-426, 2005
• 17 Cavalcante LM, "Influence of different tests used to measure the bond strength to dentin of two adhesive systems" 19 : 37-40, 2006
• 18 Adebayo OA, "Effects of conditioners on microshear bond strength to enamel after carbamide peroxide bleaching and/or casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) treatment" 35 : 862-870, 2007
• 19 Xiaojun D, "Effects of CPPACP paste on the shear bond strength of orthodontic brackets" 79 : 945-950, 2009
• 20 Woronko GA, Jr, "Effect of dentin primer on the shear bond strength between composite resin and enamel" 21 : 116-121, 1996
• 21 Reynolds EC, "Effect of casein and wheyprotein solutions on caries experience and feeding patterns of the rat" 29 : 927-933, 1984
• 22 Feigal RJ, "Clinical trial of a self-etching adhesive for sealant application: success at 24 months with Prompt L-Pop" 16 : 249-251, 2003
• 23 Cross KJ, "Casein phosphopeptides in oral health-chemistry and clinical applications" 13 : 793-800, 2007
• 24 김백일, "CPP-ACP의 소개 및 임상적 활용" 대한치과의사협회 46 (46): 139-145, 2008
• 25 Perdigao J, "Bonding characteristics of self-etching adhesives to intact versus prepared enamel" 15 : 32-42, 2003
• 26 Asselin ME, "Bond strength of a sealant to permanent enamel: evaluation of 3 application protocols" 31 : 323-328, 2009
• 27 Reynolds EC, "Anticariogenicity of calcium phosphate complexes of tryptic casein phosphopeptides in the rat" 74 : 1272-1279, 1995
• 28 Reynolds EC, "Anticariogenic complexes of amorphous calcium phosphate stabilized by casein phosphopeptides:a review" 18 : 8-16, 1998
• 29 Adebayo OA, "An SEM evaluation of conditioned and bonded enamel following carbamide peroxide bleaching and casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) treatment" 37 : 297-306, 2009
• 30 Tay FR, "Aggressiveness of self-etch adhesives on unground enamel" 29 : 309-316, 2004
• 31 Iijima Y, "Acid resistance of enamel subsurface lesions remineralized by a sugarfree chewing gum containing casein phosphopeptideamorphous calcium phosphate" 38 : 551-556, 2004
• 32 Holzmeier M, "A new generation of self-etching adhesives: comparison with traditional acid etch technique" 69 : 78-93, 2008