Dual CLAVATA3 peptides in Arabidopsis shoot stem cell signaling

다국어 초록 (Multilingual Abstract)

Plant shoot stem cell pool is constantly maintainedby a negative feedback loop through peptide-receptor mediatedsignaling pathway. CLAVATA3 (CLV3) encode a 96 aminoacidprotein which is processed to 12-amino-acid orarabinosylated 13-amino-acid peptides, acting as a ligandsignal to regulate stem cell homeostasis in the shoot apicalmeristem (SAM). Although arabinosylated 13-amino-acidCLV3 peptide (CLV3p) shows more significant bindingaffinity to its receptors and biological activities in the SAM,the physiological function of two mature forms of CLV3premained an unresolved puzzle in the past decade due to thetechnical difficulties of arabinosylation modification in thepeptide synthesis. Here, we analyzed the role of two matureCLV3 peptides with newly synthesized arabinosylated peptide.
Beside shoot meristem phenotypes, arabinosylated CLV3pshowed the conventional trait of CLV2-dependent root growthinhibition. Moreover, both 12-amino-acid and arabinosylated13-amino-acid CLV3 peptides have analogous activities inshoot stem cell signaling. Notably, we demonstrated thatnon-arabinosylated 12-amino acid CLV3p can affect shootstem cell signaling at the physiological level unlike previouslysuggested (Ohyama et al. 2009; Shinohara and Matsubayashi2013; Shinohara and Matsubayashi 2015). Therefore, theseresults support the physiological role of the 12-amino-acidCLV3p in shoot stem cell signaling in the deficient conditionof arabinosylated 13-amino-acid CLV3p in Arabidopsisthaliana.

참고문헌 (Reference)

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