플라보노이드 갤랑인의 항산화 작용에 대한 분광학적 연구 = Spectroscopic Study on the Anti-oxidant Properties of Flavonoid Galangin

다국어 초록 (Multilingual Abstract)

Purpose: The properties of the flavonoid galangin were investigated via ultravioletvisible (UV-Vis) spectroscopy to obtain further insights into its anti-oxidant behavior. Methods: A UV–Vis spectrophotometer was employed to obtain the absorption spectra of galangin. To clarify the oxidation process of galangin, its spectroscopic behavior was examined not only in different media, such as aerated and deaerated water, but also at different pH values of solution. Results: After the addition of galangin to water, the UV–Vis absorption spectra of the solution changed over time. In addition, the UV–Vis absorption spectra of galangin changed not only in an aerated neutral solution but also in a deaerated basic galangin solution, indicating the occurrence of oxidation. With an increase in the pH values of the solution, the oxidation rate of galangin increased. Spectral changes were not observed for the deaerated acidic solution. Conclusion: The UV–Vis spectroscopy results indicated that galangin is more stable in an acidic aqueous solution than in a basic solution. The reactive oxygen species (ROS) can react with both galangin as well as normal skin cells in a human body as a result of competition. The acidic form of flavonoid is acceptable when flavonoid compounds are used as skin-care products.

국문 초록 (Abstract)

목적: 본 연구에서는 항산화 기능을 갖는다고 알려진 플라보노이드 갤랑인의 항산화 특성을 분광학적 방법을 이용하여 고찰해 보고자 한다. 방법: 산성과 염기성 수용액에서의 갤랑인 분자의 흡수스펙트럼을 자외선 분광광도계를 이용하여 측정하였다. 결과: 갤랑인의 흡수스펙트럼 형태는 수용액 중에 존재하고 있는 산소나 이온화된 갤랑인 음이온의 생성에 의해 크게 변화하였는데, 이는 갤랑인이 퀴논 계열로 산화되기 때문이라는 것을 알 수 있었다. 이러한 갤랑인의 산화반응은 용액의 pH에 의존하는데, pH가 증가하여 갤랑인 음이온의 농도가 진해지면 갤랑인이 산화되는 정도는 훨씬 빨라졌다. 반면에 산소가 제거된 산성 수용액 상태에서는 산성도의 세기나 보관시간에 관계없이 흡수피크의 형태가 일정하게 유지되어 산화제가 없는 경우 산화반응이 거의 진행되지 않고 비교적 안정하게 존재할 수 있음을 확인하였다. 결론: 갤랑인과 같은 플라보노이드 화합물을 피부미용제로 이용하는 경우에는 산성 상태에서 보관하고 제조하였을 경우에 훨씬 더 안정한 상태로 존재할 수 있어 더 나은 항산화제의 역할을 할 것으로 판단된다.

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