Gas Chromatography를 이용한 세균동정법의 임상적 유용성에 관한 연구 = Clinical Feasibility Utilizing Gas Chromaatography in Microbial Identification

국문 초록 (Abstract)

본 연구의 목적은 세균동정시 병원성이라고 알려진 세균만을 주로 동정하고 그 이외의 비병원성 혹은 상재균에 속하는 균종들은 무시되어 기회감염의 원인균인지 정확히 알 수가 없었기 때문에 세균세포벽에 존재하는 지방산을 이용한 동정법을 이용하여 흔히 볼 수 없는 세균들을 검출할 수 있는 가능성을 제시하여 임상에 도움을 주는 데 그 목적이 있다.

다국어 초록 (Multilingual Abstract)

Medically important microorganisms can be identified in many ways. Conventional methods usually rely on the enzyme activity. Immunodiagnostic and nucleotide hybridization techniques have improved sensitivity, specificity, precision, but required probe and high technique.
Cellular fatty acid analysis which is one of the chemotaxonomy method is also precise and can result in the definition of high discrimmatory properties.
The prospects for using GLC of bacterial cellular fatty acids in diagnostic microbiology have been appreciated for several years in our laboratory. Whole cell fatty acid methylester(FAME) profiles of the bacteria were studied using GLC with application of the fused silica capillary column and the results were as follows :
1.Staphylococcus aureus and coagulase-negative species have qualitatively similar FAME composition, but the quantitative differencies were distinguished from each species. They consist mainly of C14 : 0 iso, C15 : 0 iso, C15 :0 anteiso, C16 : 0, C18 :0, C20 :0.
2.Comparison of FAME of 3 different genera in Enterobacteriaceae which is Proteus milabilis, Klebsiella pneumonia, Citobacter diversus were very different. Typical FAME are C16 : 0, C17 : 0 cyclo, C19 : 0 cyclo, C16 : 1 cis 9, Most also contain C12 : 0 20H, C14 : 0 20H, Cl6 : 1 C in variable amounts.
3.Comparison of FAME of 4 different Family which is Bacillus thuringiensis, Acinetobacter baumannii, Aeromonas caviae, Salmonella choleraesuis were very different and could be distinguished from each other.
In conclusion, this study provided clinical feasibility of utilizing GLC in rapid detection and Identification of several bacterial groups or species.

목차 (Table of Contents)

• Ⅰ.서론
• Ⅱ.실험재료 및 방법
• 1.사용 균주
• 2.세균 배양 조건 및 배지
• 3.시약
• 4.시험 재료
• 5.기기의 분석조건
• Ⅲ.연구결과
• 1.Calibration mixture의 chromatogram
• 2.Staphylococcus 균종들의 지방산 분포
• 3.Staphylococcus 균종들의 chromatogram
• 4.서로 다른 균속(Genus)에 속하는 세균인 Proteus milabilis, Klebsiella pneumoniae, citrobacter diversus를 비교하였다(Fig 3).
• 5.서로 다른 균과(Familuy)에 속하는 세균인 Bacillus thuringiensis, Acinetobactor baumannii, Aeromonas caviae, Salmonella choleraesuis를 비교하였다(Fig 4).
• Ⅳ.고안
• Ⅴ.결론