Trolox가 백서 태자 중뇌조직 배양에 미치는 영향 = The Effects of Trolox in Mesencephalic Tissue Cultures of Fetal Rat

다국어 초록 (Multilingual Abstract)

Parkinson's disease has pathological changes which are the depletion of dopamine producing neurons in the pars compacta of the substantia nigra. It is treated with L-dopa or the stereotactic thalamotomy which may assist to relieve the symptoms of Parkinson's disease, but often unacceptable side effects occur and such therapies can't prevent the progression of the disease.
As a new therapeutic modality, the transplantation of the dopamine producing neurons was designed and has been attempted. For the effective results of the neural transplantation, the author wanted to preserve neuronal cells more effectively. So, the author considered the use of antioxidant, alpha-tocopherol. Alpha-tocopherol was reported that it was involved in mechanisms regulating neuronal survival and neurite formation in the central nervous system. It is extremely lipophilic and taken up by cells relatively slowly. It is not an ideal therapeutic antioxidant. Trolox, a hydrophilic analogue of alpha-tocopherol was reported to scavenge peroxyl radicals better than vitamin E and penetrate biomembranes more rapidly.
The effects of Trolox on the culture of the ventral mesencephalic neurons of fetal rat were studied. Primary culture were established from 14-day fetal bain of SpragueDawley rats. The neurons were cultured on 24 wells with the media. Trolox was administrated with the concentration of 0, 0.5, 1, 1.5, 2, 3mM. More than 60% of the cells could survive for 7 days at above 1.5mM Trolox, whereas only 18% of cells were observed in its absence and development of cellular processes were markedly improved in trolox treated groups compared with that of control group. The increase of the concentration of the Trolox upto 2mM, the effect of neuronal survival were increased. So, the author concluded that Trolox was the effective modality for preservation and development of mesencephalic cells and the effect was increased as dose-dependent until 2mM. It may be involved in mechanisms regulating cellular survival and development.