해조류 청각(Codium fragile) 에탄올 추출물의 항산화에 관한 연구 = A Study on Seaweed Sea Staghorn(Codium fragile) Ethanol Extract for Antioxidant

다국어 초록 (Multilingual Abstract)

Seaweeds are rich in in minerals, vitamins, proteins, and fiber, and are classified as green algae, and they are distributed on the coasts of Korea, East Asia, Oceania, etc. and are used as a health function material as well as food ingredients in our countries and countries. In this study, Codium fragile was extracted from ethanol and concentrated to confirm DPPH radical scavenging activity, SOD activity, FRAP and ABTS cation radical scavenging ability. As a result, DPPH scavenging activity was 0.83, 22.83, 38.27, 40.93, 45.60% at 6.25, 12.50, 25, 50 and 100 μg/mL, SOD-like activity were 23.13, 33.63, 33.93, 44.07 and 59.07%. FRAP and ABTS showed antioxidant activity in a concentration-dependent manner, as in the previous experiment. Therefore, this study confirmed that it can be used as a cosmetic material using Codium fragile, a natural material.

국문 초록 (Abstract)

해조류는 무기질, 비타민, 단백질, 섬유소가 풍부하며 녹조류에 속하는 청각(Codium fragile)은 청각과이며, 우리나라, 동아시아, 오세아니아 등 해안에 분포해 있고 우리나라와 각국에서 식재료 뿐만 아니라 건강기능소재로 사용되고 있다. 본 연구에서는 청각을 주정으로 추출하고 농축하여 DPPH 라디칼 소거능, SOD 활성, FRAP과 ABTS 양이온 라디칼 소거능을 확인하였다. 그 결과 DPPH 소거능 측정 결과 6.25, 12.50, 25, 50, 100 μg/mL 농도에서 0.83, 22.83, 38.27, 40.93, 45.60%의 소거능을 나타냈으며, SOD 유사활성 측정 결과 23.13, 33.63, 33.93, 44.07, 59.07%의 유사활성능을 보였다. FRAP과 ABTS도 앞에서 했던 실험 결과와 마찬가지로 농도의존적으로 항산화 활성을 보였다. 따라서 본 연구결과에서는 천연 소재인 청각을 이용하여 화장품 소재로서 활용할 수 있음을 확인하였다.

참고문헌 (Reference)

• 1 김정환, "해양 미생물 유래 해조 다당류 분해 효소의 특성 및 산업적 응용" 한국미생물·생명공학회 39 (39): 189-199, 2011
• 2 손정우, "한국 해조류 음식의 문헌적 고찰 - 1450~1950년대를 중심으로 -" 한국식품영양학회 22 (22): 75-85, 2009
• 3 이미영, "파래가루를 첨가한 절편의 품질특성" 국제문화기술진흥원 4 (4): 295-300, 2018
• 4 최종일, "칠면초 분획물의 항산화 활성" 한국식품영양과학회 38 (38): 131-135, 2009
• 5 김민선, "추출방법에 따른 청각 및 모과 추출물의 항균활성 및 기능성" 한국생물공학회 33 (33): 89-94, 2018
• 6 심윤영, "청각으로부터 분리한 다당류의 혈액응고 저해기작 및 in vivo 항응고 활성" 한국식품영양과학회 31 (31): 917-923, 2002
• 7 신동범, "제주 연안 갈조류 추출물의 신경세포 보호효과" 한국식품영양과학회 43 (43): 224-230, 2014
• 8 신소림, "자생 양치식물 지상부와 근경 메탄올 추출물의 항산화 효과" 한국자원식물학회 23 (23): 38-46, 2010
• 9 J. M. McCord, "The utility of superoxide dismutase in studying free radical reactions. I. Radicals generated by the interaction of sulfite, dimethyl sulfoxide, and oxygen" 244 (244): 6053-, 1963
• 10 K. Kim, "Studies on the Manufacture of Undaria pinnatifida Laver and Its Physicochemical Properties-. Chemical Composition" 15 (15): 277-281, 1983
• 11 S. Gupta, "Recent developments in the application of seaweeds or seaweed extracts as a means for enhancing the safety and quality attributes of foods" 12 (12): 600-609, 2011
• 12 M. Ciancia, "Overview of anticoagulant activity of sulfated polysaccharides from seaweeds in relation to their structures, focusing on those of green seaweeds" 17 (17): 2503-2529, 2010
• 13 H. Lee, J., "Inhibitory activities of sea weeds on prolyl endopeptidase, tyrosinase and coagulation" 30 (30): 231-237, 1999
• 14 J. B. Lee, "Immunostimulating effects of sulfated galactan from Codium fragile" 345 (345): 1452-1454, 2010
• 15 D. J. Rogers, "Electron microscopy of human erythrocytes aglutinated by lectin from Codium fragile sp. tomentosoides and pseudohaemagglutinin from Ascophyllum nodosum" 3 : 83-86, 1991
• 16 Z. Chengkui, "Chinese seaweeds in herbal medicine" 116 (116): 152-154, 1984
• 17 R. Kolsi, "Characterization and evaluating of antioxidant and antihypertensive properties of green alga (Codium fragile) from the coast of Sfax" 6 (6): 186-191, 2017
• 18 Aránzazu Bocanegra, "Characteristics and Nutritional and Cardiovascular-Health Properties of Seaweeds" 한국식품영양과학회 12 (12): 236-258, 2009
• 19 이찬, "B16 멜라닌 세포에서 해양소재 추출물의 멜라닌 생성 저해 효과" 대한본초학회 27 (27): 73-80, 2012
• 20 K. J. Cho, "Antitumor effect and immunology activity of seaweeds toward sarcoma-180" 23 (23): 345-352, 1990
• 21 R. Re, "Antioxidant activity applying an improved ABTS radical cation decolorization assay" 26 (26): 1231-1237, 1999
• 22 D. J. Rogers, "Anticoagulant activity of a proteoglycan in extracts of Codium Fragile sp. atlanticum" 2 (2): 357-561, 1990
• 23 강창희, "Anti-Inflammatory Effects of Methanol Extract of Codium fragile in Lipopolysaccharide-Stimulated RAW 264.7 Cells" 한국식품영양과학회 15 (15): 44-50, 2012
• 24 Y. S. Oh, "An annotated account of Korean economic seaweeds for food, medical and industrial uses" 5 (5): 57-71, 1990
• 25 A. Cumashi, "A comparative study of the anti-inflammatory, anticoagulant, antiangiogenic and antiadhesive activities of nine different fucoidans from brown seaweeds" 17 (17): 541-542, 2007