비만세포에서 시판 간장 유래 다당류의 항염증 효과

국문 초록 (Abstract)

간장은 한국의 전통 발효식품으로써 최근 항암 효과, 항균작용, 항산화능, 항혈전 효과 등의 효능이 보고되고있다. 한국의 간장은 콩과 밀을 주요 원료로 사용한다. 콩의 세포벽으로부터 유래되는 다당류는 효소적 가수분해에 저항성이 있으므로, 발효가 끝난 후에도 간장에 잔존하게 되며 이것을 간장 유래 다당류(Kanjang polysaccharides, KPS)라 부른다. 본 연구에서는 산분해법과 양조법으로 제조된 시판 간장인 A~T의 20종의 투석물로부터 다당류를 제조하고, 비만세포에서 염증성 cytokine의 방출과 mRNA의 발현에 대한 KPS의 효과를 실험함으로써 항염증 효과를 조사하였다. RBL-2H3 세포에서 KPS의 처리는 histamine과 β-hexosaminidase의 방출을 현저히 억제시켰다. 자극된 HMC-1 세포에서 KPS의 처리는 염증성 cytokine인 IL-6, IL-8, TNF-α의 방출 및 mRNA의 발현을 감소시켰다. 특히, 양조간장으로부터 유래된 KPS는 산분해 간장보다 비만세포에서 우수한 항염증 효과를 나타내었다. 따라서, KPS는 알레르기성 염증반응을 억제시키는데 효과적일 것이라 사료된다.

다국어 초록 (Multilingual Abstract)

Soy sauce is a traditional fermented seasoning in several oriental countries, such as Korea and Japan, and recently it has been reported to have biological activities. In Korean soy sauce, soybeans and wheat are the two main raw materials. Polysaccharides that originate from the cell wall of soybeans are resistant to enzymatic hydrolysis. These polysaccharides remain in the soy sauce even after fermentation and are termed Kanjang polysaccharides (KPS). In this study, polysaccharides were obtained from dialysate of different soy sauces labeled as A~T and manufactured by fermentation or the acid-hydrolyzate method. We investigated anti-inflammatory activities by examining the effects of these KPS on proinflammatory cytokine release and mRNA expression in mast cells. Histamine and β-hexosaminidase release were strongly decreased by the KPS treatment in RBL-2H3 cells. Treatment with KPS clearly reduced mRNA expression and the release of the proinflammatory cytokines interleukin (IL)-6, IL-8, and tumor necrosis factor-alpha (TNF-α) in PMACI-stimulated HMC-1 cells. In particular, KPS derived from fermented Kanjang products showed a significant anti-inflammation effect on mast cells compared to the acid-hydrolyzed Kanjang products. This study suggests that KPS appear to be effective in suppressing allergic inflammatory reactions.

목차 (Table of Contents)

• 서론
• 재료 및 방법
• 결과 및 고찰
• 감사의 글
• References
• 초록

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