독성 물질 측정을 위한 Photobacterium phosphoreum의 고정화에 관한 연구 = Selection of Immobilization Matrix of Photobacterium phosphoreum for Detection of Toxic Substances

국문 초록 (Abstract)

독성 물질 측정용 P phosphoreum의 고정화 물질 선택을위하여 고정화 방법을 크게 4가지로 나누어서 그 방법에 따라 각각 고정화 물질 한가지씩을 선택하여 P phosphoreum의 bioluminescence 안정성을 알아보았다. Polyacrylamide나 collagen에서는 bioluminescence가 유지를 못하고 20분 안에 급격히 떨어졌으나, alginate와 k-carrageenan은 온도를 높여야 gel이 형성되는 성질을 갖고 있기 때문에 저온성 발광 미생물인 P phosphoreum에 적합한 matrix가 되지 못한다. 따라서 P phosphoreum의 biluminescence를 안정되게 유지하면서 고정화가 용이한 polymer로는 alginate가 적합한 고정화 물질로 선정되었고 독성물질에 대한 P phosphoreum의 민감성을 bioluminescence의 변화로 살펴본 결과 각각의 독성 물질마다 특이한 linearity한 관계식이 설정되었고 EC_(50)를 쉽게 구할 수 있었다.

다국어 초록 (Multilingual Abstract)

The bacterial bioluminescence has been studied to detect toxic materials m foods and environment. A monitonng system can be developed with a fact that the bioluminescence is mhibited sensitively when the bioluminescent bacteria are exposed to toxic substances. However, a stability of bioluminescence must be improved for the purpose of developing continuous monitoring system. The mmobilization of Photobacterium phosphoreum, therefore, was investigated m view of long term of bioluminescence stability. Various matrixes, sodium algmate, k-carrageenan, collagen and polyacrylamide were studied for proper immobilization matnx of P. phosphoreum, in order to improve stability of bioluminescence. Collagen and polyacrylamide were shown to be madequate matenals for immobilization of P. phosphoreum since the bioluminescence decreased when cells were mixed with such matnx due to toxic effect of free radicals of matnx. In case of k-carrageenan, the biolumexence was stable compared with collagen and polyacrylamide. However, the k-carrageenan was not also a suitable matnx because cells were not able to mix with the matnx properly m low temperature at which gel is formed and, especially, cells of P. phosphoreum are psychrophilic luminescent bacteria. With a sodium algmate, the bioluminescence was stably maintained for 20 minutes and, in addition to that, a process of immobilization was simple for bioluminescent bacteria. Therefore, by using a sodium algmate as an mobduahon matrix, the relationship between biolummescence of immobilized P. phosphoreum and each toxic substance was shown linearity and could calculate EC_(50).