Prostaglandin F_2α와 家兎의 十二指腸 Muscarinic Receptor와의 關係 = Interaction between Prostaglandin F_2 alpha and the Muscarinic Receptor on the Longitudinal Muscle of the Isolated Rabbit Duodenum

국문 초록 (Abstract)

Prostaglandin F_2α(PGF_2α)가 家兎 十二指腸 縱走筋을 收縮시킴에 있어서 acetycholine(Ach)과 같은 receptor site 에 作用하는 것인지, 아니면 다른 作用點을 가지고 있는 것인지를 알아보기 위하여 다음과 같은 實驗을 하였다.
體重 2.5∼3.0㎏ 의 健康家兎를 後頭部에 一擊을 加하여 失神케 한 後, 總頸動脈을 切斷함으로써 瀉血 犧牲시킨 卽時 腹壁을 切開하여, 十二指腸의 近位部를 摘出하고, 36.5∼37.0℃의 Tyrode 씨 營養液을 채운 magnus 槽에 懸垂하여 kymography 하였다.
懸垂괸 腸切片은 PGF_2α(5.64×10 exp(-7)M)와 Ach(1.1×10 exp(-5)M)에 의하여 tonus가 增加되었는데, 이들 兩者에 의한 tonus의 增加는 正常的인 Tyrode's 營養液內에서나, Ca-free medium 內에서 나를 莫論하고 그 程度와 pattern에 있어서 極히 類似하였다.
Atropine(1.2×10 exp(-5)M)은 Ach 에 의한 tonus 의 變化는 完全히 拮抗하였으나 PGF_2α에 의한 tonus의 變化는 전혀 拮抗하지 못하였다. 그러므로 PGF_2α는 家免의 摘出 十二指腸 縱走筋에서 그 緊張度를 增加시키기는 하나 Ach 과 같은 receptor site에 作用하는 것은 아니라고 볼 수 있다.

다국어 초록 (Multilingual Abstract)

This study aims to sustain that increased contractility of the longitudinal muscle isolated from the rabbit duodenum produced by PGF_2α is the result of the interaction of PGF_2αwith a specific action site other than the muscarinic receptor.
Healthy rabbits of 2.5-3.0kg body weight were brought into unconscious state by a blow to the head, sacrificed by cutting the carotid arteries.
The proximal segment of the duodenum was isolated and the longitudinal muscle strip was prepared for kymography in the Magnus bath filled with Tyrode's solution.
PGF_2α(5.64×10 exp(-7)M) or acetylcholine(ACH; 1.1×10 exp(-5)M) was added separately into the medium after the spontaneous contractile activity became regular.
The muscle tone was increased by the addition of both PGF_2α and ACH, and the characteristics of tonus induced by PGF_2α was quite similar to that induced by ACH both in the normal and Ca-free medium.
Atropine(1.2×10 exp(-5)M) abolished the contraction produced by ACH, but the contraction produced by PGF_2α was unaffected in the presence of atropine.
These results suggest that PGF_2α does not interact with the muscarinic receptor under the conditions employed in this study, but has other site of action.