구굴무치과에 속하는 동사리와 얼룩동사리의 Carotenoid 색소성분의 비교

국문 초록 (Abstract)

어류의 carotenoid에 관한 비교 생화학적 연구의 일환으로서, 우리나라 특산의 담수어인 구굴무치과의 동사리와 얼룩동사리의 표피 carotenoid를 TLC, column chromatography 및 HPLC로 분리하고 NaBH₄에 의한 환원반응, I₂에 의한 이성화반응 및 uv-visible spectrophotometer로 비교하고 IR, ¹H-NMIR 및 mass spectrum을 측정하여 carotenoid를 동정한 결과는 다음과 같다. 동사리 표피의 총 carotenoids 함량은 4윌산에 3.01㎎%, 9월산에 3.67㎎%로서 산란기에 가까워질수록 높게 나타났고, carotenoid 조성은 4월산에 β-carotene(25.6%), lutein(18.5%), zeaxanthin(12.0%)이 주성분을 이루고, 그 외 isocryptoxanthin, diatoxanthin, tunaxanthin, cynthiaxanthin, canthaxanthin 및 α-cryptoxanthin을 소량 성분으로 함유하였으며, 9월산에는 β-carotene(16.5%), zeaxanthin(13.7%) cynthiaxanthin(13.6%)이 주성분을 이루며, 그 외 lutein, isocryptoxanthin, tunaxanthin, α-cryptoxanthin, diatoxanthin 및 canthaxanthin을 소량 성분으로 함유하여, 산란기에 가까워질수록 cynthiaxanthin과 α-cryptoxanthin의 조성이 증가하는 것을 볼 수 있었다. 얼룩동사리 표피의 총 carotenoids 함량은 4월산에 2.00㎎%, 9월산에 2.84㎎%로서 산란기에 가까워질수록 높게 나타났고, carotenoid 조성은 4월산과 9월산 모두에서 β-carotene(24.9%, 27.5%), zeaxanthin(14.4%, 20.9%), lutein(l2.6%, 11.4%)이 주성분을 이루고, 그 외 cynthiaxanthin, tunaxanthin, diatoxanthin, isocryptoxanthin, α-cryptoxanthin 및 canthaxanthin을 소량 성분으로 함유하여, 산란기에 가까워질수록 zeaxanthin의 조성이 증가하는 것을 볼 수 있었으며, 동사리와 비교하여 서로 다르게 나타났다. 이러한 결과는 같은 구굴무치과이면서도 서식 환경 및 표피 색체의 차이에 기인하는 것으로 보아진다. 그리고 동사리와 얼룩동사리는 다른 담수어에서는 드물게 볼 수 있는 cynthiaxanthin과 diatoxanthin의 함량이 높게 함유하며 해산어 특유의 tunaxanthin도 함유하여 매우 특이하였다.

다국어 초록 (Multilingual Abstract)

This study was performed as a part of comparative biochemical studies of carotenoid pigment for the fresh water fish. Carotenoids in integument of Korean dark sleeper, Odontobutis platycephala, and dark sleeper, Odontobutis odontobutis interrupta, which are all the Korean native fresh water fish, were separated by thin layer chromatography. column chromatography and HPLC. The separated carotenoid were then reduced and isomerized by NaBH₄ and I₂ respectively to investigate UV-Vis spectrophotometeric patterns and characterized by IR, ¹H-NMR and Mass spectrum. The content of total carotenoids in the integument of Korean dark sleeper was 3.01㎎% in April, but it was increased to 3.74㎎% in September at the near of spawning period. The carotenoid isolated in April consisted of β-carotene(25.6%), lutein(18.5%) and zeaxanthin(12.0%) as major carotenoids and also contained isocryptoxanthin, diatoxanthin, tunaxanthin, cynthiaxanthin, canthaxanthin and α-cryptoxanthin as minor carotenoids. Similarly, in September the carotenoid consisted of β-carotene(16.5%), zeaxanthin(13.7%) and cynthiaxanthin(13.6%) as major carotenoids and also contained lutein, isocryptoxanthin, tunaxanthin, α-cryptoxanthin, diatoxanthin and canthaxanthin as minor carotenoids. At the near of spawning period, the content of cynthiaxanthin and α-cryptoxanthin were increased. The content of total carotenoids in the integument of dark sleeper was 2.00㎎% in April but it was increased to 2.84㎎% in September at the near of spawning period. The carotenoid isolated in April and September consisted of β-carotene(24.9%, 27.5%), zeaxanthin(14.4%, 20.9%) and lutein(l2.6%, 11.4%) as major carotenoids and also contained cynthiaxanthin, tunaxanthin, diatoxanthin, isocryptoxanthin, α-cryptoxanthin and canthaxanthin as minor carotenoids. At the near of spawning period, the content of zeaxanthin was increased, indicating that the carotenoid composition were dependent upon their living conditions and their integument colors. Both Korean dark sleeper and dark sleeper contained high amount of cynthiaxanthin and diatoxanthin which are found as rare carotenoids in the other of fresh water fish. It is interest that they also contained tunaxanthin which is a specific carotenoid in marine fishes.

목차 (Table of Contents)

• Abstract
  
• 서론
  
• 재료 및 방법
  
• 결과 및 고찰
  
• 요약
  
• 문헌