블랙베어 및 어소금의 잎 색 변화에 따른 생리활성 물질 변화 = Changes of biologically active compounds in Echeveria spp. and Adromischus maculatus depending on leaf color changes

국문 초록 (Abstract)

블랙베어 (Echeveria spp.)와 어소금 (Adromischus maculatus)은 빛과 온도에 따라 색상이 변화하는 관상용 다육식물이다. 이들의 잎 색상은 관상 가치와 시장 수요에 중요한 역할을 하지만, 다육식물 잎에 함유된 플라보노이드에 대한 정량적 연구는 부족한 실정이다. 본 연구에서는 블랙베어와 어소금의 색상 변화와 내부 생리활성 물질 간의 연관성을 파악하기 위해 색도계와 초고성능 액체 크로마토그래피 (UPLC)를 활용하여 잎 표면 색상 변화와 생리활성 물질 성분을 비교 분석하였다. 색도계 분석 결과, 블랙베어는 초기 대비 적색이 42.31%, 녹색이 49.32%, 청색이 21.58% 증가하며 잎 색상이 검붉은색에서 녹색으로 뚜렷하게 변하였다. 반면, 어소금의 경우 적색은 0.15%, 녹색은 10.44% 감소하여 녹색의 감소폭이 더 컸고, 이로 인해 녹색에서 붉은 점박이 패턴이 더욱 두드러지고 면적이 넓어졌다. UPLC 분석에서는 블랙베어 잎 추출물에서 quercitrin이, 어소금에서는 quercetin-3-O-sambubioside가 검출되었다. 이러한 물질들은 환경변화 (빛, 온도)에 따라 안토시아닌 농도와 색 변화에 주요하게 작용하는 것으로 판단된다. 본 연구는 다육식물의 빛과 온도에 따른 잎 색 변화와 생리활성 물질의 상관성을 이해하는 데 기초 자료로 활용될 수 있을 것으로 기대된다.

다국어 초록 (Multilingual Abstract)

Black bear (Echeveria spp.) and Adromischus maculatus are ornamental succulents that change color in response to light and temperature. Colors of leaves play an important role in their ornamental value and market demand. However, there is a lack of quantitative research on flavonoids contained in succulent leaves. In this study, a colorimeter and ultra-performance liquid chromatography (UPLC) were used to analyze color changes of leaf surfaces and components of bioactive substances to understand the relationship between color change and internal bioactive substances of black bear and A. maculatus. The colorimeter analysis showed that red, green, and blue colors of black bear increased by 42.31%, 49.32%, and 21.58%, respectively, compared to their initial values, resulting in a clear change in leaf color from reddish-black to green. On the other hand, in A. maculatus, the red color decreased by 0.15% and the green color decreased by 10.44%, resulting in a more pronounced red spotted pattern and an increased area. UPLC analysis detected quercitrin in Black Bear leaf extracts and quercetin-3-O-sambubioside in A. maculatus. These substances are considered to play a key role in the concentration of anthocyanins and color changes in response to environmental changes such as light and temperature. Results of this study could be used as basic data to understand the correlation between leaf color change and bioactive substances in succulents in response to light and temperature.

참고문헌 (Reference)

• 1 Lee JS, "Year-round leaf color changes of several Sedum species" Korean Society for Horticultural Science 27 (27): 148-, 2009
• 2 Wrolstad RE, "Tracking color and pigment changes in anthcyanin products" 16 : 423-428, 2005
• 3 최스란 ; 김유선, "The analysis of plant color change according to the changing growing environment of indoor wall planting space" 9 : 107-115, 2014
• 4 Erwin J, "Temperature impacts cactus and succulent development rate" 27 : 65-68, 2017
• 5 Yoshida K, "Synchrony between flower opening and petal-color change from red to blue in morning glory, Ipomoea tricolor cv. Heavenly Blue" 85 : 187-197, 2009
• 6 Chen J, "Relationship between anthocyanin composition and floral color of Hibiscus syriacus" 9 : 48-, 2023
• 7 Vargas-Álvarez D, "Quercetin, kaempferol and apigenin in roselle (Hibiscus sabdariffa L.)" 5 : 62-66, 2018
• 8 박예지 ; 김기철 ; 조상훈 ; 문선애 ; 김한택 ; 정아용 ; 김아람 ; 채경석 ; 김지원 ; 박명기, "Quercetin and quercetin glycoside contents in colored foods" 52 : 750-757, 2023
• 9 Li M, "Proteomics reveal the profiles of color change in Brunfelsia acuminata flowers" 20 : 2000-, 2019
• 10 Chávez-Reyes Y, "Polyphenol oxidase inactivation by microwave oven and Its effect on phenolic profile of loquat (Eriobotrya japonica) fruit" 4 : 87-94, 2013
• 11 Vignolini S, "Pointillist structural color in Pollia fruit" 109 : 15712-15715, 2012
• 12 Im DJ, "Numerical study on the iridescence of multi-level diffraction gratings" 97-, 2014
• 13 Pervaiz T, "Naturally occurring anthocyanin, structure, functions and biosynthetic pathway in fruit plants" 5 : 1000187-, 2017
• 14 Airoldi CA, "Mechanical buckling can pattern the light-diffracting cuticle of Hibiscus trionum" 36 : 109715-, 2021
• 15 Ma Y, "Leaf color and growth change of Sedum rubrotinctum caused by two commercial chemical products" 54 : 434-444, 2019
• 16 Seo HB, "Large-scale photonic crystal structures mimicking the structural color of organisms" Korean Society of Mechanical Engineers 654-659, 2012
• 17 Deng K, "Improved stability of blue colour of anthocyanins from Lycium ruthenicum murr. based on copigmentation" 27 : 6089-, 2022
• 18 Jing L, "Identification and quantification of flavonoids in 207 cultivated lotus (Nelumbo nucifera) and their contribution to different colors" 2022
• 19 Delazar A, "Free-radical scavenging flavonol 3-O-glycosides from the leaves of Ribes biebersteinii Berl" 4 : 96-100, 2010
• 20 Matlawska I, "Flavonoid compounds in the flowers of Kitaibelia vitifolia willd. (Malvaceae)" 58 : 127-132, 2001
• 21 Azuma A, "Flavonoid biosynthesis-related genes in grape skin are differentially regulated by temperature and light conditions" 236 : 1067-1080, 2012
• 22 이상용 ; 김영희 ; 이지은 ; 황은경, "Evaluation of the discoloration of Pyropia yezoensis Ueda using a colorimeter" 36 : 620-625, 2018
• 23 Raisa Aone M. Cabahug ; 소손일 ; 남상용, "Effects of shading on the growth, development, and anthocyanin content of Echeveria agavoides and E. marcus" 25 : 270-277, 2017
• 24 Raisa Aone M. Cabahug ; 소손일 ; 남상용, "Effects of light intensity on the growth, development, and anthocyanin content of Echeveria agavoides and E. marcus" 25 : 262-269, 2017
• 25 Zhao LQ, "Effects of cell morphology, physiology, biochemistry and CHS genes on four flower colors of Impatiens uliginosa" 15 : 1343830-, 2024
• 26 황방연 ; 남상영 ; 김인재 ; 김민자 ; 윤태 ; 박성규 ; 이우영 ; 홍성수, "Effect on qualities of italic as degree of sunlight exposure after harvest" 18 : 505-511, 2005
• 27 박필만 ; 이수영 ; 박부희 ; 안혜련 ; J. Ryan Stewart ; Héctor Genaro Ortiz Cano, "Effect of the different pH levels of nutrient solution on the growth and color of Echeveria ‘Perle von Nurnberg’" 29 : 199-205, 2021
• 28 Cortell JM, "Effect of shading on accumulation of flavonoid compounds in (Vitis vinifera L.) Pinot noir fruit and extraction in a model system" 54 : 8510-8520, 2006
• 29 Li J, "Composition and antioxidant activity of anthocyanins and non-anthocyanin flavonoids in blackberry from different growth stages" 11 : 2902-, 2022
• 30 Chen J, "Chemistry, pharmacokinetics, pharmacological activities, and toxicity of quercitrin" 36 : 1545-1575, 2022
• 31 Teppabut Y, "Change of petals‘color and chemical components in Oenothera flowers during senescence" 23 : 1698-, 2018
• 32 이나영 ; 이종석 ; 한승원, "Application to RGB Image data for analysis of leaf color in succulent plants" 9 : 84-94, 2006
• 33 Deng S, "Application of high-speed counter-current chromatography coupled with high performance liquid chromatography for the separation and purification of Quercetin-3-O-sambubioside from the leaves of Nelumbonucifera" 3 : 375-382, 2009
• 34 Ortega-Regules A, "Anthocyanin fingerprint of grapes: Environmental and genetic variations" 86 : 1460-1467, 2006
• 35 박성익 ; 황희영 ; 김종식 ; 박승우, "Analysis of the functional components and various biological activities of Korean medicinal herbs" 48 : 1345-1351, 2019
• 36 Rodgerson C, "Adromischus" 12 : 42-45, 1994
• 37 Zheng J, "A systematic investigation on free phenolic acids and flavonoids profiles of commonly consumed edible flowers in China" 172 : 268-277, 2019
• 38 Choi J, "A study of succulent home gardening assistance system based on deep learning" 28 : 697-699, 2021
• 39 Smith CA, "A review of the genus Adromischus Lemaire" 3 : 613-651, 1939