국내 황칠나무 발효 추출물의 항균력 평가

국문 초록 (Abstract)

본 연구에서는 Lactobacillus plantarum (L. plantarum) ilchiwhangchil 1785와 L. plantarum ilchiwhangchil 2020을 이용하여 황칠나무 잎·가지 추출물 및 수액의 발효 조건을 검토하였다. 지수성장기의 L. plantarum ilchiwhangchil 1785와 L. plantarum ilchiwhangchil 2020 균주를 발효에 사용하였다. 발효 중에 발효액의 pH 변화와 생균수의 변화를 분석하였다. 그 결과, 적당한 발효 조건은 잎·가지 추출물 20 wt% 및 수액 1 wt%를 함유하고, 37℃에서 2일간 발효시키는 것이었다. 잎 · 가지, 수액 발효 추출물의 항균력은 최소저해농도법(MIC)과 disk diffusion 방법을 이용하여 대장균(Escherichia coli, E. coli), 포도상구균(Staphylococcus aureus, S. aureus), 녹농균(Pseudomonas aeruginosa, P. aeruginosa)을 대상으로 비교하였다. 비발효 추출물을 함유한 배지에서 배양한 것보다 황칠나무 잎 · 가지 추출물 및 수액의 발효액을 함유한 배지에서 배양한 세 가지 균주 모두에서 항균활성이 증가하였다. 또한, 황칠나무 발효 추출물의 함량에 비례하여 항균 활성이 증가하였다. 연구 결과는 황칠나무 잎 · 가지 추출물 및 수액의 발효물의 천연보존제, 화장품소재 및 천연물 포장소재 등 천연 생기능성 물질로의 응용가능성을 시사한다.

다국어 초록 (Multilingual Abstract)

We investigated the fermentation conditions for extracts of leave/branches and sap from Korean Dendropanax morbifera (D. morbifera) using Lactobacillus plantarum (L. plantarum) ilchiwhangchil 1785 and L. plantarum ilchiwhangchil 2020. Log growth phase cultured L. plantarum ilchiwhangchil 1785 and L. plantarum ilchiwhangchil 2020 were used for fermentation. The pH and growth of the microorganisms in broth were monitored during the fermentation period. The results revealed that the optimum fermentation conditions for 20 wt% of leave/branches extracts and 1 wt% of sap extract was 2 days incubation at 37℃. The minimum inhibitory concentration (MIC) method and a disk diffusion assay were used to evaluate the antimicrobial activity of the fermented extracts of the leave/branches and sap against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. The antimicrobial activity increased in all three strains grown on the medium containing fermented extracts of the leave/branches and sap as compared with that of the strains grown on medium containing non fermented extracts. Furthermore, the antimicrobial activity increased in proportion to the contents of the fermented extracts. Our data suggest that fermented extracts of leave/branches and sap of D. morbifera have applications as natural bio functional materials, such as preservatives, cosmetic materials, and natural packaging materials.

목차 (Table of Contents)

• 서론
• 재료 및 방법
• 결과 및 고찰
• References
• 초록

참고문헌 (Reference)

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