Plasmid 分析에 依한 院內感染의 疫學的 調査 = Molecular Epidemiology of Nosocomial Infection by Plasmid Analysis

국문 초록 (Abstract)

R plasmid 傳達에 依해 蔓延된 耐性菌은 抗菌劑로 充滿된 病院環境에서 棲息하기에 有利하다. 이들 耐性菌에 依해 발생한 院內感染은 抗菌劑選擇의 어려움이 따른다. 院內感染의 集團發生을 疫學的으로 규명하는데는 在來式 菌種同定方法으로는 限界가 있기 때문에 분자생물학적 방법으로 plasmid를 분석한다. plasmid profile ?? plasmid fingerprinting 과 restriction endo-unclease analysis 및 DNA-DNA hybridization 등의 分子的 特性化(molecular characterization)와 비적합성군(incompatibility group)을 조사하는 유전적 특성화(genetic characterization)의 방법들은 菌種에 관계없이 共通으로 存在하는 R plasmid들의 同一性 或은 類似性을 把握하면 그 由來를 추적할 수 있으므로 耐性菌에 관한 분자생물학적 疫學調査方法에 있어 決定的인 資料가 된다.

다국어 초록 (Multilingual Abstract)

Due to the selective pressure of hospital environment saturated with antimicrobid agents, multipIy resistant strains harbor- R plasmids evoke mmplexity in treahent of nosoGoTniaL infections. For the rational effort to control outbreaks of nosucornial infections, new systems for epidemioIogica1 studies had been developed. In recent years, molecular epidemioIogy have been introduced to investigate the epidemics cam& by R plasSmid cadexring multiple resistanf; strains as well as hospital associated infectjon. Malecular epidemiology can be classified with two cakgories of genetic characterization and molecular characterization. Genetic chracterization includes the test for resistznCe transfer and identification of R plasmids by incornpat gronping with standard ref erence strains. Molecular characterization means molecular biologid technique including plzsmid fingerprinting by gel electrophoresis with or without restrictior endonuclease analysis, DNA-DNA hybridization, and heteroduplex analysis, to, confirm the degree of homoIogy between diferent plasmids regardless their originating bacterial species and identify the trapusable sequence. These techniques ma be used to identify epidemk strains and epidemic plasmids which have spread through several different bacterial species. The approach to the invpigation of outbreaks of hospital-associated infections and epidemics can be illustrated by a number of examples; incompatibility groups of Gram negagve enteric bacteria isolated in Korea, plasmid profiles of gel electrophoresis and restridon endonuclease analpis of possible causatim agents in nosocornid infection.