전자선 조사한 건조 멸치류에서 유도된 지방분해산물의 분석

국문 초록 (Abstract)

건조된 국멸치와 지리멸치 및 밴댕이에 1, 3, 5, 7 및 10 k㏉의 선량으로 전자선을 조사하여 비조사 시료와 조사된 시료에서 지방을 추출한 후 전자선 조사 시 유도 생성되는 hydrocarbon류를 florisil column chromatography를 이용하여 분리하였으며, hydrocarbon류의 함량 및 분해패턴은 GC/MS로 확인하였다. 전자선 조사된 건조 멸치류에서 palmitic acid로부터 생성되는 1-tetradecene(C14:1), pentadecane(C15:0)과 stearic acid로부터 1-hexadecene(C16:1), heptadecane(C17:0) 및 oleic acid로부터 8-heptadecene (C17:1), 1,7-hexadecadiene(C16:2)이 확인되었으며, 6종의 hydrocarbon류는 조사선량에 따라 증가하였다. 건조된 멸치류에서 유기용매에서 기인한 것으로 판단되는 pentadecane(C15:0) 및 heptadecane(C17:0)을 제외한 hydrocarbon류 중 8-heptadecene(C17:1)의 함량이 가장 높았으며, 다음으로 1-tetradecene(C14:1), 1-hexadecene(C16:1), 1,7-hexadecadiene(C16:2) 순으로 확인되었다. 따라서 전자선 조사된 건조 멸치류에서 분석된 hydrocarbon류 중 1-tetradecene(C14:1), 1-hexadecene(C16:1) 그리고 8-heptadecene(C17:1) 및 1,7-hexadecadiene(C16:2)은 전자선 조사 여부를 판별할 수 있는 유용한 지표로 활용될 수 있을 것이라고 사료된다.

다국어 초록 (Multilingual Abstract)

Radiation-induced hydrocarbon contents of dried anchovy, jiri anchovy, and large-eyed herring were evaluated following electron-beam irradiation at doses of 1, 3, 5, 7, and 10 k㏉. GC/MS identification of the induced hydrocarbons by irradiation was conducted after lipid separation by soxtec, followed by florosil column chromatography. 1-Tetradecene (C14:1) and pentadecane (C15:0) derived from palmitic acid, 1-hexadecene (C16:1) and heptadecane (C17:0) from stearic acid, and 8-heptadecene (C17:1) and 1,7-hexadecadiene (C16:2) from oleic acid were the major induced hydrocarbons in irradiated dried anchovy, jiri anchovy, and large-eyed herring samples. At the same irradiation dose, concentration of induced hydrocarbons differed from fatty acid composition and increased in accordance with radiation dose level. Radiation-induced hydrocarbons, such as 1-tetradecene (C14:1), 1-hexadecene (C16:2), 8-heptadecene (C17:1), and 1,7-hexadecadiene (C16:2), were confirmed as irradiation marker compounds. Therefore, these marker compounds could be used to distinguish electron-beam irradiated dried anchovy, jiri anchovy, and large-eyed herring from non-irradiated ones.

목차 (Table of Contents)

• ABSTRACT
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• REFERENCES

참고문헌 (Reference)

• 1 추효상, "하계 한국 남해의 해황 변동과 멸치 초기 생활기 분포특성" 한국수산과학회 35 (35): 77-85, 2002
• 2 윤미혜, "조미건어포류의 안전성에 대한 조사연구" 한국식품위생안전성학회 24 (24): 143-147, 2009
• 3 심성례, "전자선 조사한 캠벨얼리 포도(Vitis labrusca L.)의 휘발성 유기성분 변화" 한국식품저장유통학회 17 (17): 151-159, 2010
• 4 이해정, "건조 홍합의 방사선 조사여부를 확인하기 위한 지방분해산물 분석" 한국식품영양과학회 31 (31): 599-603, 2002
• 5 노정은, "건멸치의 방사선 조사 확인을 위한 열발광, 전자스핀공명, Hydrocarbon 및 2-Alkylcyclobutanone의 다중분석" 한국식품영양과학회 32 (32): 8-14, 2003
• 6 권중호, "감마선 조사와 저장 기간에 따른 건멸치류의 품질 및 열발광 특성" 한국식품저장유통학회 9 (9): 19-27, 2002
• 7 박병준, "감마선 조사와 저염함량으로 제조한 된장의 미생물 및 품질특성 변화" 한국식품과학회 34 (34): 79-84, 2002
• 8 Lee EH, "The taste compounds in boiled-dried anchovy" 14 : 194-200, 1981
• 9 Lee EH, "The effect of Taipet-F and Bactokil on retarding lipid oxidation in boiled-dried anchovy" 18 : 181-188, 1989
• 10 Chung HW, "The application of different detection methods for irradiated dried anchovy and shrimp" 63 : 411-414, 2002
• 11 Ham HJ, "Sulfites contents survey on dried marine products in market" 14 : 380-385, 1999
• 12 Metcalf LD, "Rapid preparation of fatty acid esters from lipid for gas chromatographic analysis" 38 : 514-515, 1996
• 13 Chan DC, "Randomized controlled trial of the effect of n-3 fatty acid supplementation on the metabolism of apolipoprotein B-100 and chylomicron remnants in men with visceral obesity" 77 : 300-307, 2003
• 14 Kim KS, "Quantitative comparison of radiation-induced hydrocarbons from irradiated beef, pork and chicken" 31 : 301-307, 1999
• 15 Pyeun JH, "Proteolytic properties of cathepsin L, chymotrypsin, and trypsin from the muscle and viscera of anchovy, Engraulis japonica" 28 : 557-568, 1995
• 16 Lee EH, "Nutritional component and health in the fishery resources of the coastal and offshore waters in Korea" Youil Publishing Co. 43-46, 1977
• 17 NFRD, "National fisheries research development institute homepage"
• 18 Takiguchi A, "Lipid oxidation and hydrolysis in dried anchovy products during drying and storage" 53 : 1463-1469, 1987
• 19 KFDA., "Korea Food Standard Code 2012-48" Korea Food & Drug Administration 2-3, 2012
• 20 IAEA, "International atomic energy agency homepage"
• 21 Kwon JH, "Gamma irradiation combined with improved packaging for preserving and improving the quality of dried fish(Engraulis encrasicholus)" 46 : 725-729, 1995
• 22 Kim JS, "Food component characteristics of plain-dried anchovies on the market" 29 : 20-25, 2000
• 23 Kim IS, "Food component characteristics of cold air dried anchovies" 29 : 973-980, 2000
• 24 Dallongeville J, "Fish consumption is associated with lower heart rates" 108 : 820-825, 2003
• 25 Lee EH, "Fatty acid content of five kinds of boiled-dried anchovies on the market" 19 : 183-186, 1986
• 26 Lee KH, "Effects of packing on the quality stability and shelf-life of dried anchovy" 14 : 229-234, 1985
• 27 Jo KS, "Effect of packaging method on the storage stability of boiled-dried anchovy" 19 : 195-199, 1987
• 28 Jeong BY, "Effect of deoxygenizer on the suppression of lipid deterioration of boiled and dried-anchovy, Engraulis japonica. Ⅱ. Changes in n-3 polyunsaturated fatty acids" 28 : 779-792, 1995
• 29 Jeong BY, "Effect of deoxygenizer on the suppression of lipid deterioration of boiled and dried-anchovy, Engraulis japonica. Ⅰ. Changes in lipid class compositions" 28 : 770-778, 1995
• 30 Delincée H, "Detection of food treated with ionizing radiation" 9 : 73-82, 1998
• 31 Lee EH, "Degradation of acid soluble nucleotides and their related compounds in seafoods during processing and storage" 4 : 31-41, 1971
• 32 Hwang KT, "Application of hydrocarbons as markers for detecting post-irradiation of imported meats and fish" 26 : 1109-1115, 1997
• 33 Byun MW, "Application and aspect of irradiation technology in food industry" 30 (30): 89-100, 1997