흰 생쥐의 태아기 저단백식이가 생장후의 운동능력 및 젓산형성에 미치는 영향 = The Effect of Low Protein Diet During Pregnancy on the Physical Performance and Lactate Formation in Child Mice

다국어 초록 (Multilingual Abstract)

The effect of maternal protein deficiency during gestation and lactation on the physical performance and lactate formation of the child mice was studied. Thirthy female mice were devided into two groups consisting of 15 each, one group was maintained with balanced and the other with low protein diet. The former, the control group, was fed balanced diet containing 20% casein and the latter, experimental group was fed low protein diet containing 5% casein during the periods of gestation, delivery and lactation. 30 male mice weaned from each group (Total 60 mice) were fed with balanced diet until the experiment. 15 mice from each group (total 30 mice) was used for single acute experiment and the other half were trained under severe training program. 8 weeks after weaning, the maximal running time was recorded on a small animal treadmill with a speed of 34.3m/min and 19° slope. 3 minutes after the maximal running, the mouse was sacrificed immediately by decaptitation and the blood was collected. The lactic and pyruvic acid in the blood were determined by the method of Barker and Summerson (1941), and Friedman and Haugen (1942) respectively.
The results were summarized as follows;
1. Newly born young mice from low protein group during gestation and lactation gained less weight than balanced diet group until 8 weeks after weaning.
2. The maximal running time of the low protein diet group was longer than control group significantly (P<0.05). The control group ran for an average of 7.23±1.46 min compared to 11.53±1.28 min of experimental group and the maximal work done of experimental group was also higher than control, 4238±470 gm-m and 2698±544 gm-m respectively.
3. The lactate concentration and lactate/pyruvate ratio in blood of the control group was higher then the experimental group.
4. The maximal running time and the maximal work of both groups after severe training program became insignificant, although the experimental group showed a longer maximal time.