배양된 내피세포의 Endothelin, PDGF 및 PGI₂의 분비능에 관한 실험적 연구 = The Effects of Angiotensin II and Thrombin on the Secretion of PDGF Endothelin, and PGI₂in Cultured Umbilical Vein Endothelial Cells

다국어 초록 (Multilingual Abstract)

The endothelial cell plays an important role in physiologic hemostasis, blood vessel permeability, and the response of the vessel to other physiologic and pathologic stimuli. Abnor- malities of endothelial cell structure and function may contribute significantly to disease of blood vessel wall such as thromobsis, atherosclerosis, and vasculitis. The role of endothelial cells in these important biologic events might be better defined if it were possible to isolate and culture them for in vitro study.
Culture of these cells has been attempted by many investigators over the last few years, but two recurring problems have hampered this work: firstly, inability to maintain endothelial cells in pure culture for reasonable periods of time; secondly, inability to identify the cultured cells as endothelial cell.
This study was proceeded to reveal the optimal media condition of the growth of endothelial cells which were obtained from human umbilical vein of neonate by a sterile technique. When these cells were cultured in Media 199 containing 20% fetal calf serum, heparin 10 U/ml, endothelial cell growth factor(ECGF) 5 ng/ml, and penicillin-streptomycin 1%, the mean population doubling time was 97.7 hours. When l% of bovine pituitary extract(BPE) was added in that media, that was 51.3 hours. Conclusively, endothelial cells were cultured more rapidly by BPE, and a certain growth factor for endothelial cell might be present in BPE.
This study also confirm the secretion of PDGF, endothelin, and prostacyclin by cultured umbilical vein endothelial jcells, and the secretory activity of prostacyclin was increased significantly by angiotensin II and thrombin, and that of endothelin was increased significantly by thrombin. The stimulative effect of endothelial secretion was more profound thrombin was added.