Capillaria hepatica 感染白鼠에 있어서 蟲卵切片 抗原에 대한 間接螢光抗體反應에 관한 硏究 = Indirect fluorescent antibody test on the sectioned egg antigen of Capillaria hepatica in the liver of white rat

다국어 초록 (Multilingual Abstract)

A study on the indirect fluorescent antibody test(IFAT) using the sectioned egg antigen in the liver of white rats was performed.
The antigen used was prepared in 6~8㎛ thickness by frozen section at -20℃ of nonembryonated C. hepatica eggs deposited in the liver tissue after 6 weeks of infection. Fluorescein isothiocyanate (FITC) conjugated anti-rat IgG (Goat) was diluted by 64 fold in phosphate buffered saline (PBS, pH 7.2) and counterstaining with a 1/10,000 solution of Evans blue was effected while the fluorescent conjugate is acting by diluting the labelled antiglobulin in the Evans blue solution instead of in the PBS.
A total of 22 white rats (B.W. 150g) was divided into 2 groups (A, B) and 200 and 2,000 embryonated eggs were infected orally. All rats were bled by retro-orbital puncture prior to infection and weeks of 1, 3, 5, 7, 9 and 13 after infection. As negative control sera, 60 normal rats were used. Eggs for infection were cultured at room temparature for 4~5 weeks in 0.5% formalin. IFAT was processed by the modified method of Wilson et al. (1974).
The results obtained were summarized as follows;
1. The localization of antigens in frozen section of non-embryonated eggs of C. hepatica detected by IFAT was seen on the inner membrane of egg shell.
2. IgG titers detected by IFAT rapidly increased in 3 weeks after infection, thereafter the titers were kept until 5 weeks and gradually decreased in 7 weeks to 9 weeks, then converted into normal by 13 weeks.
3. IgG titers distributed from 16 to 1,024 and showed highest titers in 3 and 5 weeks.
4. Mean titers of 3, 5, 7, 9 weeks after infection were higher in group B than in group A.